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TBST (or TTBS) is mixture of Tris-Buffered Saline and Tween 20. It is a buffer used for washing nitrocellulose membrane in western blotting and microtiter plate wells in ELISA assays.

Contents of TBS-Tween

The following is a sample recipe for TBST:

Adjust pH with HCl to pH 7.6

The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water.[1]


  1. ^ Medicago AB, (2010) Tris buffered saline with Tween pH 7.6 specification sheet