From Wikipedia, the free encyclopedia - View original article

Stevia rebaudiana flowers
Scientific classification

About 240 species, including:

Jump to: navigation, search
Stevia rebaudiana flowers
Scientific classification

About 240 species, including:

Stevia (/ˈstvɪə/, /ˈstvjə/ or /ˈstɛvɪə/)[1][2][3][4] is a genus of about 240 species of herbs and shrubs in the sunflower family (Asteraceae), native to subtropical and tropical regions from western North America to South America. The species Stevia rebaudiana, commonly known as sweetleaf, sweet leaf, sugarleaf, or simply stevia, is widely grown for its sweet leaves. As a sweetener and sugar substitute, stevia's taste has a slower onset and longer duration than that of sugar, and some of its extracts may have a bitter or licorice-like aftertaste at high concentrations.

With its steviol glycoside extracts having up to 300 times the sweetness of sugar,[5] stevia has attracted attention with the rise in demand for low-carbohydrate, low-sugar sweeteners. Because stevia has a negligible effect on blood glucose it is attractive to people on carbohydrate-controlled diets.

The availability of stevia varies from country to country. In a few countries, it has been available as a sweetener for decades or centuries; for example, it has been widely used for decades as a sweetener in Japan. In some countries health concerns and political controversies have limited its availability; for example, the United States banned stevia in the early 1990s unless labeled as a dietary supplement,[6][7] but in 2008 it approved rebaudioside A extract as a food additive. Over the years, the number of countries in which stevia is available as a sweetener has been increasing. In 2011, stevia was approved for use in the EU.[8][9]

History and use[edit]

The genus Stevia consists of 240[10] species of plants native to South America, Central America, and Mexico, with several species found as far north as Arizona, New Mexico, and Texas.[11] The genus was named for Spanish botanist and physician Petrus Jacobus Stevus (Pedro Jaime Esteve 1500–1556) author of commentaries on Nicander and Hippocrates and of an unpublished flora of the Kingdom of Valencia.[12] Human use of the sweet species S. rebaudiana originated in South America. The leaves of the stevia plant have 30–45 times the sweetness of sucrose (ordinary table sugar).[13] The leaves can be eaten fresh, or put in teas and foods.

The plant has a long history of medicinal use by the Guaraní, having been used extensively by them for more than 1,500 years.[14] The leaves have been traditionally used for hundreds of years in both Brazil and Paraguay to sweeten local teas and medicines, and as a "sweet treat".[14]

Steviol is the basic building block of stevia's sweet glycosides.

In 1899 Swiss botanist Moisés Santiago Bertoni, while conducting research in eastern Paraguay, first described the plant and the sweet taste in detail.[15] Only limited research was conducted on the topic until in 1931 two French chemists isolated the glycosides that give stevia its sweet taste.[16] These compounds, stevioside and rebaudioside, are 250–300 times as sweet as sucrose and are heat-stable, pH-stable, and not fermentable.[5]

The exact structure of the aglycone and the glycoside was published in 1955.

In the early 1970s, sweeteners such as cyclamate and saccharin were suspected of being carcinogens. Consequently, Japan began cultivating stevia as an alternative. The plant's leaves, as well as the aqueous extract of the leaves and purified steviosides, were developed as sweeteners. The first commercial stevia sweetener in Japan was produced by the Japanese firm Morita Kagaku Kogyo Co., Ltd. in 1971.[17] The Japanese have been using stevia in food products and soft drinks, (including Coca Cola),[18] and for table use. Japan currently consumes more stevia than any other country, with stevia accounting for 40% of the sweetener market.[19]

Today, stevia is cultivated and used to sweeten food elsewhere in East Asia including China (since 1984), Korea, Taiwan, Thailand, and Malaysia. It can also be found in Saint Kitts and Nevis, Brazil, Colombia, Peru, Paraguay, Uruguay, and Israel. China is the world's largest exporter of stevioside.[19]

Stevia species, which are found in the wild in semiarid habitats ranging from grassland to mountain terrain, do produce seeds, but only a small percentage of the seeds germinate. Planting cloned stevia is a more effective method of reproduction.[citation needed]

Folk medicine and research[edit]

For centuries, the Guaraní peoples of Paraguay used stevia, which they called ka'a he'ê ("sweet herb"), as a sweetener in yerba mate and other foods, and medicinally as a cardiac stimulant and as a treatment for obesity, hypertension and heartburn, and to help lower uric acid levels.[20] Current research has evaluated its effects on obesity[21] and hypertension.[22][23] Since stevia has been found to have a negligible effect on blood glucose and may even enhance glucose tolerance,[24] it may be useful as a natural sweetener for diabetics and others on carbohydrate-controlled diets.[25]


The stevia plant may be grown legally in most countries, although some countries restrict its use as a sweetener.
Stevia as widely sold in health food stores in Germany
Widely used as a sweetener
Available as a food additive (Regulatory agency approved)
Available as both a food additive and dietary supplement
Available (regulatory status unverified)
Stevia extract on sale in a supermarket in Paraguay

Availability notes[edit]


Steviol glycosides were first commercialized as a sweetener in 1971 by the Japanese firm Morita Kagaku Kogyo Co., Ltd., a leading stevia extract producer in Japan.

Stevia has been grown on an experimental basis in Ontario, Canada since 1987 to determine the feasibility of commercial cultivation.

In 2007 The Coca-Cola Company announced plans to obtain approval for their stevia-derived sweetener, rebiana, for use as a food additive within the United States by 2009, as well as plans to market rebiana-sweetened products in 12 countries that allow stevia's use as a food additive.[41][42] In May 2008 Coca Cola and Cargill announced the availability of Truvia, a consumer brand stevia sweetener containing erythritol and rebiana,[43] which the FDA permitted as a food additive in December 2008.[44] Coca-Cola announced intentions to release stevia-sweetened beverages in late December 2008.[45]

Shortly afterward, PepsiCo and Pure Circle announced PureVia, their brand of stevia-based sweetener, but withheld release of beverages sweetened with rebaudioside A until receipt of FDA confirmation. Since the FDA permitted Truvia and PureVia, both Coca Cola and PepsiCo have introduced products that contain their new sweeteners.[46]

Extraction of sweet compounds[edit]

S. rebaudiana foliage

Rebaudioside A has the least bitterness of all the steviol glycosides in the stevia plant. To produce rebaudioside A commercially, stevia plants are dried and subjected to a water extraction process. This crude extract contains about 50% rebaudioside A; its various glycoside molecules are separated via crystallization techniques, typically using ethanol or methanol as solvent. This allows the manufacturer to isolate pure rebaudioside A.[47]

The National Research Council of Canada has patented a process for extracting sweet compounds from stevia by column extraction at temperatures from 0 to 25 °C, followed by purification by nanofiltration. A microfiltration pretreatment step is used to clarify the extract. Purification is by ultrafiltration followed by nanofiltration.[48]

Mechanism of action[edit]

Glycosides are molecules that contain glucose and other non-sugar substances called aglycones (molecules with other sugars are polysaccharides). The tongue's taste receptors react to the glucose in the glycosides – those with more glucose (rebaudioside) taste sweeter than those with less (stevioside).[49] Some of the tongue's bitter receptors react to the aglycones.

In the digestive tract, rebaudiosides are metabolised into stevioside. Then stevioside is broken down into glucose and steviol. The glucose released in this process is used by bacteria in the colon and not absorbed into the bloodstream.[50] Steviol cannot be further digested and is excreted.

Health and safety[edit]

Stevia leaves have been used for centuries in South America, spanning multiple generations in ethnomedical tradition as a treatment for diabetes mellitus type 2.[51] Millions of Japanese have been using stevia for over thirty years with no reported or known harmful effects.[52]

In 2012, the FDA stated that it has not permitted the use of whole-leaf Stevia or crude Stevia extract citing possible concerns on blood sugar and effects on the reproductive, cardiovascular, and renal systems.[53] In 2009 the FDA considered "Rebiana (rebaudioside A) to be generally recognized as safe (GRAS)".[54] The report includes a detailed list of international studies, references, and chemical analysis.[54]

Two 2010 review studies found no health concerns with stevia or its sweetening extracts.[55][56] In addition, a 2009 review study found that stevioside and related compounds have anti-hyperglycemic, anti-hypertensive, anti-inflammatory, anti-tumor, anti-diarrheal, diuretic, and immunomodulatory actions.[57]

The European Food Safety Authority evaluated the safety of steviol glycosides, extracted from the leaves of the Stevia rebaudiana Bertoni plant, as sweetener and expressed its opinion on 10 March 2010. The Authority established an Acceptable Daily Intake (ADI) for steviol glycosides, expressed as steviol equivalents, of 4 mg/kg bodyweight/day. On 11 November 2011, the European Commission allowed the usage of steviol glycosides as a food additive, establishing maximum content levels for different types of foods and beverages.[58]

Preliminary human studies suggest stevia may lower blood pressure,[59] although another study has shown it to have no effect on hypertension.[60]

In 2006 the World Health Organization (WHO) evaluated experimental studies of stevioside and steviols conducted on animals and humans, and concluded "stevioside and rebaudioside A are not genotoxic in vitro or in vivo and that the genotoxicity of steviol and some of its oxidative derivatives in vitro is not expressed in vivo."[61] The WHO also found no evidence of carcinogenic activity. Furthermore, the report noted "stevioside has shown some evidence of pharmacological effects in patients with hypertension or with diabetes mellitus type 2,"[61] but concluded further study was required to determine proper dosage. The WHO's Joint Experts Committee on Food Additives has approved, based on long-term studies, an acceptable daily intake of steviol glycoside of up to 4 milligrams per kilogram of body weight.[62]

A 1985 study reported that steviol, a breakdown product from stevioside and rebaudioside (two of the sweet steviol glycosides in the stevia leaf), is a mutagen in the presence of a liver extract of rats pretreated with a PCB blend[63] – but this finding was criticized.[64] Over the following years, bioassay, cell culture, and animal studies have shown mixed results in terms of toxicology and adverse effects of stevia constituents. While reports emerged that found steviol and stevioside to be weak mutagens,[65][66] the bulk of studies show an absence of harmful effects.[67][68] In a 2008 review, 14 of 16 studies cited showed no genotoxic activity for stevioside, 11 of 15 studies showed no genotoxic activity for steviol, and no studies showed genotoxicity for rebaudioside A. No evidence for stevia constituents causing cancer or birth defects has been found.[67][68]

In relation to diabetes, studies have shown stevia to have a possible trophic effect on β-cells of pancreas,[14] to improve insulin sensitivity in rats,[69] and possibly even to promote additional insulin production,[70] helping to reverse diabetes and metabolic syndrome.[71] Stevia consumed before meals significantly reduced postprandial insulin levels compared to both aspartame and sucrose.[72] A 2011 review study stated that stevia sweeteners would likely benefit diabetic patients.[55] In 2013 a study with stevia leaves on diabetic rats identified a renal and hepatic protective effect and a reduction in the risk of oxidative stress, in addition to the better known hypoglycemic benefits.[73]


In 1991, after receiving an anonymous industry complaint, the United States Food and Drug Administration (FDA) labeled stevia as an "unsafe food additive" and restricted its import.[40][74][75] The FDA's stated reason was "toxicological information on stevia is inadequate to demonstrate its safety."[76]

Since the import ban in 1991, marketers and consumers of stevia have shared a belief that the FDA acted in response to industry pressure.[40] Arizona congressman Jon Kyl, for example, called the FDA action against stevia "a restraint of trade to benefit the artificial sweetener industry".[77] To protect the complainant, the FDA deleted names in the original complaint in its responses to requests filed under the Freedom of Information Act.[40]

Stevia remained banned until after the 1994 Dietary Supplement Health and Education Act forced the FDA in 1995 to revise its stance to permit stevia to be used as a dietary supplement, although not as a food additive – a position that stevia proponents regarded as contradictory because it simultaneously labels stevia as safe and unsafe, depending on how it is sold.[6]

Early studies prompted the European Commission in 1999 to ban stevia's use in food in the European Union pending further research.[78] In 2006, research data compiled in the safety evaluation released by the World Health Organization found no adverse effects.[61] Since 2008, the Russian Federation has allowed stevioside as a food additive "in the minimal dosage required".[31]

In December 2008, the FDA gave a "no objection" approval for GRAS status to Truvia (developed by Cargill and The Coca-Cola Company) and PureVia (developed by PepsiCo and the Whole Earth Sweetener Company, a subsidiary of Merisant), both of which use rebaudioside A derived from the Stevia plant.[79] However, FDA said that these products are not Stevia, but a highly purified product.[80]

In 2012, FDA posted a note on their website regarding crude Stevia plant: "FDA has not permitted the use of whole-leaf Stevia or crude Stevia extracts because these substances have not been approved for use as a food additive. FDA does not consider their use in food to be GRAS in light of reports in the literature that raise concerns about the use of these substances. Among these concerns are control of blood sugar and effects on the reproductive, cardiovascular, and renal systems."[81]

See also[edit]


  1. ^ "Stevia". 2012-08-31. Retrieved 2013-02-13. 
  2. ^ "Stevia". British & World English. 2013-02-07. Retrieved 2013-02-13. 
  3. ^ "Stevia". US English. 2013-02-07. Retrieved 2013-02-13. 
  4. ^ Both /ˈstvɪə/ and /ˈstɛvɪə/ are recorded by at least some US and UK dictionaries, but the former is more common in US English (listed first or exclusively) and the latter is more common in UK English.
  5. ^ a b Raji Akintunde Abdullateef, Mohamad Osman (2012-01-01). "Studies on effects of pruning on vegetative traits in Stevia rebaudiana Bertoni (Compositae)". International Journal of Biology 4 (1). doi:10.5539/ijb.v4n1p146. 
  6. ^ a b McCaleb, Rob (1997). "Controversial Products in the Natural Foods Market". Herb Research Foundation. Retrieved 8 November 2006. 
  7. ^ "Dietary Supplement Health and Education Act of 1994". 2011. Retrieved 31 January 2011. 
  8. ^ a b Lucas, Louise (2011). "Brussels backs Stevia sweetener". Financial Times. Retrieved 22 November 2011. 
  9. ^ a b Stones, Mike (2011). "Stevia wins final EU approval". Retrieved 22 November 2011. 
  10. ^ "Stevia". Flora of North America. 
  11. ^ "Stevia Cav.". USDA Plants. 
  12. ^ Parsons, WT; Cuthbertson, EG (2001). Noxious Weeds of Australia, 2nd ed.. Collingswood, Australia: CSIRO Publishing. ISBN 978-0-643-06514-7.  This reference refers specifically to Stevia eupatoria, a related weed having the same nomenclature origin.
  13. ^ "Opinion on Stevia Rebaudiana plants and leaves" (PDF) (Press release). European Commission Scientific Committee on Food. 17 June 1999. Retrieved 27 January 2008. 
  14. ^ a b c Misra, H.; Soni, M.; Silawat, N.; Mehta, D.; Mehta, B. K.; Jain, D. C. (Apr 2011). "Antidiabetic activity of medium-polar extract from the leaves of Stevia rebaudiana Bert. (Bertoni) on alloxan-induced diabetic rats". J Pharm Bioallied Sci 3 (2): 242–8. doi:10.4103/0975-7406.80779. PMC 3103919. PMID 21687353. 
  15. ^ Bertoni, Moisés Santiago (1899). Revista de Agronomia de l'Assomption 1: 35. 
  16. ^ Bridel, M.; Lavielle, R. (1931). "Sur le principe sucre des feuilles de kaa-he-e (stevia rebaundiana B)". Academie des Sciences Paris Comptes Rendus (Parts 192): 1123–5. 
  17. ^ "Stevia". Morita Kagaku Kogyuo Co., Ltd. 2004. Retrieved 6 November 2007. 
  18. ^ Taylor, Leslie (2005). The Healing Power of Natural Herbs. Garden City Park, NY: Square One Publishers, Inc. pp. (excerpted at weblink). ISBN 0-7570-0144-0. 
  19. ^ a b Jones, Georgia (September 2006). "Stevia". NebGuide: University of Nebraska–Lincoln Institute of Agriculture and Natural Resources. Retrieved 4 May 2007. 
  20. ^ Tanvir, Ashraf (24 May 2005). "Sugar Leav – A new breed of 'sweetener'". Pakistan Agricultural Research Council. Retrieved 2 January 2009. 
  21. ^ "PubMed research articles related to treatments of obesity". Retrieved 2013-02-13. 
  22. ^ "PubMed research articles on stevia's effects on blood pressure". Retrieved 2013-02-13. 
  23. ^ "PubMed articles on stevia's use in treating hypertension". Retrieved 2013-02-13. 
  24. ^ Curi R, Alvarez M, Bazotte RB, Botion LM, Godoy JL, Bracht A (1986). "Effect of Stevia rebaudiana on glucose tolerance in normal adult humans". Braz. J. Med. Biol. Res. 19 (6): 771–4. PMID 3651629. 
  25. ^ Gregersen S, Jeppesen PB, Holst JJ, Hermansen K (January 2004). "Antihyperglycemic effects of stevioside in type 2 diabetic subjects". Metab. Clin. Exp. 53 (1): 73–6. doi:10.1016/j.metabol.2003.07.013. PMID 14681845. 
  26. ^ a b c d "Truvia timeline". Archived from the original on 2010-01-01. 
  27. ^ "Stevia gets Australian approval for food and beverages". Retrieved 2013-02-13. 
  28. ^ "Cap 132U Schedule (Sweeteners in Food Regulations; Public Health and Municipal Services Ordinance) |". 2011. Retrieved 22 June 2011. 
  29. ^ "Stevia Sweeteners Now Approved in Israel". 2012. Retrieved 5 April 2012. 
  30. ^ "Norwegian Stevia fact sheet Norwegian Institute of Public Health". 1999-06-17. doi:10.2903/j.efsa.2010.1537. Retrieved 2013-02-13. 
  31. ^ a b "Technical regulations for juice products from fruits and vegetables". Russian Federation Federal Law. 27 October 2008. p. Table 5. 
  32. ^ "Sale of Food Act, Chapter 283, Section 56(1) – Food Regulations". Agri-Food & Veterinary Authority of Singapore. 2005. 
  33. ^ Li, Simon (27 March 2002). Fact Sheet: Stevioside (PDF). Hong Kong Legislative Council Secretariat Research and Library Services Division. 
  34. ^ "Notice of Modification to the List of Permitted Sweeteners to Enable the Use of Steviol Glycosides as a Table-Top Sweetener and as a Sweetener in Certain Food Categories - Document Reference Number NOM/ADM-0002". Health Canada. 2012. 
  35. ^ Halliday, Jess (8 September 2009). "France approves high Reb A stevia sweeteners". Retrieved 23 January 2010. 
  36. ^ Halliday, Jess (15 September 2009). "France's first stevia products around the corner". Retrieved 23 January 2010. 
  37. ^ a b c Curry,Leslie Lake. "Agency Response Letter GRAS Notice No. GRN 000287". Retrieved 28 August 2010. 
  38. ^
  39. ^ a b c "Olam and Wilmar in 50:50 JV to Acquire 20% Stake in PureCircle, a Leading Producer of Natural High-Intensity Sweeteners for USD 106.2 Mln". 1 July 2008. Retrieved 5 April 2012. 
  40. ^ a b c d Hawke, Jenny (February–March 2003). "The Bittersweet Story of the Stevia Herb" (PDF). Nexus magazine 10 (2). Retrieved 20 December 2010. 
  41. ^ Stanford, Duane D. (31 May 2007). "Coke and Cargill teaming on new drink sweetener". Atlanta Journal-Constitution. Archived from the original on 30 September 2007. Retrieved 31 May 2007. 
  42. ^ Etter, Lauren and McKay, Betsy (31 May 2007). "Coke, Cargill Aim For a Shake-Up In Sweeteners". Wall Street Journal. Retrieved 1 June 2007. 
  43. ^ "Truvia ingredients". Retrieved 15 May 2008. 
  44. ^ "Stevia sweetener gets US FDA go-ahead". Decision News Media SAS. 18 December 2008. Retrieved 11 May 2009. 
  45. ^ "Coke to sell drinks with stevia; Pepsi holds off". The Seattle Times. Associated Press. 15 December 2008. Retrieved 16 December 2008. 
  46. ^ "FDA Approves 2 New Sweeteners". The New York Times. Associated Press. 17 December 2008. Retrieved 11 May 2009. 
  47. ^ Purkayastha, S. ""A Guide to Reb-A", Food Product Design". Retrieved 28 March 2009. 
  48. ^ "United States Patent 5,972,120 Extraction of sweet compounds from Stevia rebaudiana Bertoni". 
  49. ^ Growing Stevia, Retrieved November 7, 2013
  50. ^ Koyama, E., et al. "In vitro metabolism of the glycosidic sweeteners, stevia mixture and enzymatically modified stevia in human intestinal microflora." Food and Chemical Toxicology 41.3 (2003) 359–374.
  51. ^ Abudula R, Jeppesen PB, Rolfsen SE, Xiao J, Hermansen K (October 2004). "Rebaudioside A potently stimulates insulin secretion from isolated mouse islets: studies on the dose-, glucose-, and calcium-dependency". Metab. Clin. Exp. 53 (10): 1378–81. doi:10.1016/j.metabol.2004.04.014. PMID 15375798. 
  52. ^ "Products and Markets – Stevia". Food and Agriculture Organization of the United Nations – Forestry Department. Retrieved 4 May 2007. 
  53. ^
  54. ^ a b New York Medical College (15 January 2009). "Notice to the U.S. Food and Drug Administration (FDA) that the use of Rebiana (Rebaudioside A) derived from Stevia rebaudiana, as a Food Ingredient is Generally Recognized as Safe (GRAS)" (PDF). p. 75. Retrieved 23 August 2010. "the observed LD50 values were 5.2 g/kg bw for male hamsters and 6.1 g/kg bw for female hamsters" 
  55. ^ a b Goyal, S. K.; Samsher; Goyal, R. K. (Feb 2010). "Stevia (Stevia rebaudiana) a bio-sweetener: a review". Int J Food Sci Nutr 61 (1): 1–10. doi:10.3109/09637480903193049. PMID 19961353. 
  56. ^ Ulbricht, C.; Isaac, R.; Milkin, T.; Poole, E. A.; Rusie, E. et al. (Apr 2010). "An evidence-based systematic review of stevia by the Natural Standard Research Collaboration". Cardiovasc Hematol Agents Med Chem 8 (2): 113–27. PMID 20370653. 
  57. ^ Chatsudthipong, V.; Muanprasat, C. (Jan 2009). "Stevioside and related compounds: therapeutic benefits beyond sweetness". Pharmacol Ther 121 (1): 41–54. doi:10.1016/j.pharmthera.2008.09.007. PMID 19000919. 
  58. ^ "Commission Regulation (EU) No 1131/2011" (PDF). Official Journal of the European Union: 7. 11 November 2011. Retrieved 15 November 2011. "The CE regulation establishes steviol glycosides as food additive, and establishes maximum content levels in foodstuff and beverages." 
  59. ^ Hsieh MH, Chan P, Sue YM, et al. (November 2003). "Efficacy and tolerability of oral stevioside in patients with mild essential hypertension: a two-year, randomized, placebo-controlled study". Clin Ther 25 (11): 2797–808. doi:10.1016/S0149-2918(03)80334-X. PMID 14693305. 
  60. ^ Ferri LA, Alves-Do-Prado W, Yamada SS, Gazola S, et al. (September 2006). "Investigation of the antihypertensive effect of oral crude stevioside in patients with mild essential hypertension". Phytother Res 20 (9): 732–6. doi:10.1002/ptr.1944. PMID 16775813. 
  61. ^ a b c Benford, D. J.; DiNovi, M., Schlatter, J. (2006). "Safety Evaluation of Certain Food Additives: Steviol Glycosides" (PDF). WHO Food Additives Series (World Health Organization Joint FAO/WHO Expert Committee on Food Additives (JECFA)) 54: 140. 
  62. ^ Joint FAO/WHO Expert Committee on food additives, Sixty-ninth Meeting. World Health Organization. 4 July 2008. 
  63. ^ Pezzuto JM, Compadre CM, Swanson SM, Nanayakkara D, Kinghorn AD (April 1985). "Metabolically activated steviol, the aglycone of stevioside, is mutagenic". Proc. Natl. Acad. Sci. U.S.A. 82 (8): 2478–82. doi:10.1073/pnas.82.8.2478. PMC 397582. PMID 3887402. 
  64. ^ Procinska E, Bridges BA, Hanson JR (March 1991). "Interpretation of results with the 8-azaguanine resistance system in Salmonella typhimurium: no evidence for direct acting mutagenesis by 15-oxosteviol, a possible metabolite of steviol". Mutagenesis 6 (2): 165–7. doi:10.1093/mutage/6.2.165. PMID 2056919.  – article text is reproduced here [1].
  65. ^ Matsui M, Matsui K, Kawasaki Y, et al. (November 1996). "Evaluation of the genotoxicity of stevioside and steviol using six in vitro and one in vivo mutagenicity assays". Mutagenesis 11 (6): 573–9. doi:10.1093/mutage/11.6.573. PMID 8962427. 
  66. ^ Nunes AP, Ferreira-Machado SC, Nunes RM, Dantas FJ, et al. (2007). "Analysis of genotoxic potentiality of stevioside by comet assay". Food Chem Toxicol 45 (4): 662–6. doi:10.1016/j.fct.2006.10.015. PMID 17187912. 
  67. ^ a b Geuns JM (2003). "Stevioside". Phytochemistry 64 (5): 913–21. doi:10.1016/S0031-9422(03)00426-6. PMID 14561506. 
  68. ^ a b Brusick DJ (2008). "A critical review of the genetic toxicity of steviol and steviol glycosides". Food Chem Toxicol 46 (7): S83–S91. doi:10.1016/j.fct.2008.05.002. PMID 18556105. 
  69. ^ Lailerd N, Saengsirisuwan V, Sloniger JA, Toskulkao C, Henriksen EJ (January 2004). "Effects of stevioside on glucose transport activity in insulin-sensitive and insulin-resistant rat skeletal muscle". Metab. Clin. Exp. 53 (1): 101–7. doi:10.1016/j.metabol.2003.07.014. PMID 14681850. 
  70. ^ Jeppesen PB, Gregersen S, Rolfsen SE, et al. (March 2003). "Antihyperglycemic and blood pressure-reducing effects of stevioside in the diabetic Goto-Kakizaki rat". Metab. Clin. Exp. 52 (3): 372–8. doi:10.1053/meta.2003.50058. PMID 12647278. 
  71. ^ Dyrskog SE, Jeppesen PB, Colombo M, Abudula R, Hermansen K (September 2005). "Preventive effects of a soy-based diet supplemented with stevioside on the development of the metabolic syndrome and type 2 diabetes in Zucker diabetic fatty rats". Metab. Clin. Exp. 54 (9): 1181–8. doi:10.1016/j.metabol.2005.03.026. PMID 16125530. 
  72. ^ Anton, S. D.; Martin, C. K.; Han, H.; Coulon, S.; Cefalu, W. T., et al. (Aug 2010). "Effects of stevia, aspartame, and sucrose on food intake, satiety, and postprandial glucose and insulin levels". Appetite 55 (1): 37–43. doi:10.1016/j.appet.2010.03.009. PMC 2900484. PMID 20303371. 
  73. ^ Shivanna, Naveen; Naika, Mahadev; Khanum, Farhath; Kaul, Vijay (Mar 2013). "Antioxidant, anti-diabetic and renal protective properties of Stevia rebaudiana". Journal of Diabetes and its Complications 27 (2): 103–113. doi:10.1016/j.jdiacomp.2012.10.001. PMID 23140911. 
  74. ^ "Celestial Seasonings: Who sent the trade complaint that started the raid?".  – memorandum from the Department of Health & Human Services to its Denver office.
  75. ^ "Artificial Sweetener Controversies From Saccharin to Sucralose". Retrieved 20 December 2010. 
  76. ^ Food and Drug Administration (1995, rev 1996, 2005). Import Alert #45-06: "Automatic Detention of Stevia Leaves, Extract of Stevia Leaves, and Food Containing Stevia"
  77. ^ Kyl, John (R-Arizona) (1993). Letter to former FDA Commissioner David Aaron Kessler about the 1991 stevia import ban, quoted at safety studies.
  78. ^ European Commission Scientific Committee on Food (June 1999). Opinion on Stevioside as a Sweetener
  79. ^ Newmarker, Chris (18 December 2008). "Federal regulators give OK for Cargill's Truvia sweetener". Minneapolis / St. Paul Business Journal. Retrieved 18 December 2008. 
  80. ^ "What refined Stevia preparations have been evaluated by FDA to be used as a sweetener?". 
  81. ^ "Is Stevia an 'FDA approved' sweetener?".