SgrS (sugar transport-related sRNA, previously named ryaA) is a 227 nucleotide small RNA that is activated by SgrR in Escherichia coli during glucose-phosphate stress. The nature of glucose-phosphate stress is not fully understood, but is correlated with intracellular accumulation of glucose-6-phosphate. SgrS helps cells recover from glucose-phosphate stress by base pairing with ptsGmRNA (encoding the glucose transporter) and causing its degradation in an RNase E dependent manner. Base pairing between SgrS and ptsGmRNA also requires Hfq, an RNA chaperone frequently required by small RNAs that affect their targets through base pairing. The inability of cells expressing sgrS to create new glucose transporters leads to less glucose uptake and reduced levels of glucose-6-phosphate. SgrS is an unusual small RNA in that it also encodes a 43 amino acid functional polypeptide, SgrT, which helps cells recover from glucose-phosphate stress by preventing glucose uptake. The activity of SgrT does not affect the levels of ptsG mRNA of PtsG protein. It has been proposed that SgrT exerts its effects through regulation of the glucose transporter, PtsG.
^Vanderpool, CK; Gottesman S (2004). "Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system". Mol Microbiol54 (4): 1076–1089. doi:10.1111/j.1365-2958.2004.04348.x. PMID15522088.Cite uses deprecated parameters (help)
^Kawamoto H, Koide Y, Morita T, Aiba H (2006). "Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq". Mol. Microbiol.61 (4): 1013–22. doi:10.1111/j.1365-2958.2006.05288.x. PMID16859494.
Vanderpool CK (2007). "Physiological consequences of small RNA- mediated regulation of glucose-phosphate stress". Current Opinion in Microbiology10 (2): 1–6. doi:10.1016/j.mib.2007.03.011. PMID17383224.
Sun, Y; Vanderpool, CK (2013 Nov). "Physiological consequences of multiple-target regulation by the small RNA SgrS in Escherichia coli.". Journal of bacteriology195 (21): 4804–15. PMID23873911.Cite uses deprecated parameters (help);Check date values in: |date= (help)