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This is applicable to laboratory analysis particularly in the determination of water pollution. Such an analytical test may be either qualitative or quantitative.
A Quantitative method to determine the amount of phosphate present in samples such as boiler feedwater is as follows. A measured amount of boiler water is poured into a mixing tube and ammonium heptamolybdate reagent is added. The tube is then stoppered and vigorously shaken. The next step is to add dilute stannous chloride reagent, which has been freshly prepared from concentrated stannous chloride reagent and distilled water, to the mixture in the tube. This will produce a blue color (due to the formation of molybdenum blue) and the depth of the blue color indicates the amount of phosphate in the boiler water. The absorbance of the blue solution can be measured with a colorimeter and the concentration of phosphate in the original solution can be calculated. Alternatively, a direct (but approximate) reading of phosphate concentration can be obtained by using a Lovibond comparator.
This method for phosphate determination is known as Deniges' method. A simple Qualitative method to determine the presence of phosphate ions in a sample is as follows. A small amount of the sample is acidified with Concentrated Nitric Acid to which a little ammonium molybdate is added. The presence of Phosphate Ions is indicated by the formation of a bright yellow layer.