Influenza A virus

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Orthomyxoviridae
Electron micrograph of influenza A viruses
Virus classification
Group:Group V ((-)ssRNA)
Family:Orthomyxoviridae
Genera

Influenzavirus A
Influenzavirus B
Influenzavirus C
Isavirus
Thogotovirus

 
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Orthomyxoviridae
Electron micrograph of influenza A viruses
Virus classification
Group:Group V ((-)ssRNA)
Family:Orthomyxoviridae
Genera

Influenzavirus A
Influenzavirus B
Influenzavirus C
Isavirus
Thogotovirus

Influenza A virus causes influenza in birds and some mammals, and is the only species of influenza virus A. Influenza virus A is a genus of the Orthomyxoviridae family of viruses. Strains of all subtypes of influenza A virus have been isolated from wild birds, although disease is uncommon. Some isolates of influenza A virus cause severe disease both in domestic poultry and, rarely, in humans.[1] Occasionally, viruses are transmitted from wild aquatic birds to domestic poultry, and this may cause an outbreak or give rise to human influenza pandemics.[2][3]

Influenza A viruses are negative-sense, single-stranded, segmented RNA viruses. The several subtypes are labeled according to an H number (for the type of hemagglutinin) and an N number (for the type of neuraminidase). There are 18 different H antigens (H1 to H18) and 10 different N antigens (N1 to N10).[4] H17 was isolated from fruit bats in 2012.[5][6][7] H18 was discovered in a Peruvian bat in 2013.[8]

Each virus subtype has mutated into a variety of strains with differing pathogenic profiles; some are pathogenic to one species but not others, some are pathogenic to multiple species.

A filtered and purified influenza A vaccine for humans has been developed, and many countries have stockpiled it to allow a quick administration to the population in the event of an avian influenza pandemic. Avian influenza is sometimes called avian flu, and colloquially, bird flu. In 2011, researchers reported the discovery of an antibody effective against all types of the influenza A virus.[9]

Variants and subtypes[edit]

Influenza type A viruses are categorized into subtypes based on the type of two proteins on the surface of the viral envelope:

H = hemagglutinin, a protein that causes red blood cells to agglutinate.
N = neuraminidase, an enzyme that cleaves the glycosidic bonds of the monosaccharide, neuraminic acid

Different influenza viruses encode for different hemagglutinin and neuraminidase proteins. For example, the H5N1 virus designates an influenza A subtype that has a type 5 hemagglutinin (H) protein and a type 1 neuraminidase (N) protein. There are 18 known types of hemagglutinin and 10 known types of neuraminidase, so, in theory, 180 different combinations of these proteins are possible.[4]

Some variants are identified and named according to the isolate they resemble, thus are presumed to share lineage (example Fujian flu virus-like); according to their typical host (example human flu virus); according to their subtype (example H3N2); and according to their deadliness (example LP, low pathogenic). So a flu from a virus similar to the isolate A/Fujian/411/2002(H3N2) is called Fujian flu, human flu, and H3N2 flu.

Variants are sometimes named according to the species (host) in which the strain is endemic or to which it is adapted. The main variants named using this convention are:

Variants have also sometimes been named according to their deadliness in poultry, especially chickens:

Most known strains are extinct strains. For example, the annual flu subtype H3N2 no longer contains the strain that caused the Hong Kong flu.

Annual flu[edit]

The annual flu (also called "seasonal flu" or "human flu") in the U.S. "results in approximately 36,000 deaths and more than 200,000 hospitalizations each year. In addition to this human toll, influenza is annually responsible for a total cost of over $10 billion in the U.S."[10]

The annually updated, trivalent influenza vaccine consists of hemagglutinin (HA) surface glycoprotein components from influenza H3N2, H1N1, and B influenza viruses.[11]

Measured resistance to the standard antiviral drugs amantadine and rimantadine in H3N2 has increased from 1% in 1994 to 12% in 2003 to 91% in 2005.

"Contemporary human H3N2 influenza viruses are now endemic in pigs in southern China and can reassort with avian H5N1 viruses in this intermediate host."[12]

F16 antibody[edit]

F16, an antibody that targets the hemagglutinin protein, was recently discovered, and is the only known antibody effective against all 16 subtypes of the influenza A virus.[13][14][15]

Structure and genetics[edit]

"The physical structure of all influenza A viruses is similar. The virions or virus particles are enveloped and can be either spherical or filamentous in form. In clinical isolates that have undergone limited passages in eggs or tissue culture, there are more filamentous than spherical particles, whereas passaged laboratory strains consist mainly of spherical virions."[16]

The Influenza A virus genome is contained on eight single (non-paired) RNA strands that can code for up to 14 proteins (HA, NA, NP, M1, M2, M42, NS1, NEP, PA, PA-X, PB1, PB1-F2, PB1-N40, and PB2). The total genome size is 13,588 bases.[17] The segmented nature of the genome allows for the exchange of entire genes between different viral strains during cellular cohabitation. The eight RNA segments are:

The genome segments have common terminal sequences, and the ends of the RNA strands are partially complementary, allowing them to bond to each other by hydrogen bonds. After transcription from negative-sense to positive-sense RNA the +RNA strands get the cellular 5' cap added by cap snatching, which involves the viral protein NS1 binding to the cellular pre-mRNAs. The cap is then cleaved from the cellular pre-mRNA using a second viral protein, PA. The short oligo cap is then added to the influenza +RNA strands, allowing its processing as messenger RNA by ribosomes. The +RNA strands also serve for synthesis of -RNA strands for new virions.

The RNA synthesis and its assembly with the nucleoprotein takes place in the cell nucleus, the synthesis of proteins takes place in the cytoplasm. The assembled virion cores leave the nucleus and migrate towards the cell membrane, with patches of viral transmembrane proteins (hemagglutinin, neuraminidase and M2 proteins) and an underlying layer of the M1 protein, and bud through these patches, releasing finished enveloped viruses into the extracellular fluid.

Evidence has been found for the presence of RNA structure in Influenza A virus.[19] Structured RNA appears to be favored in the +RNA, and is clustered near regions with functional importance. Two pseudoknot RNA structures were discovered in the influenza A Segment 7 splice site and in the influenza A segment 8 (Influenza virus pseudoknot), which are both alternatively spliced to produce the M2 and NEP proteins respectively. Towards the 5' splice sites of these mRNAs, hairpin loop and multibranch loop structures were predicted. As well as these structures near splice sites, a pseudoknot was proposed in Segment 2, which encompasses the start codon for an internally transcribed open reading frame for a small polypeptide product (PB1-F2).

Multiplicity Reactivation[edit]

Influenza virus is able to undergo multiplicity reactivation after inactivation by UV radiation,[20][21] or by ionizing radiation.[22] If any of the eight RNA strands that make up the genome contains a damage that prevents replication or expression of an essential gene the virus is not viable when it alone, infects a cell (a single infection). However, when two or more damaged viruses infect the same cell (multiple infection), viable progeny viruses can be produced provided each of the eight genomic segments is present in at least one undamaged copy. That is, multiplicity reactivation can occur.

Upon infection, influenza virus induces a host response involving increased production of reactive oxygen species, and this can damage the virus genome.[23] If, under natural conditions, virus survival is ordinarily vulnerable to the challenge of oxidative damage, then multiplicity reactivation is likely selectively advantageous as a kind of genomic repair process. It has been suggested that multiplicity reactivation involving segmented RNA genomes may be similar to the earliest evolved form of sexual interaction in the RNA world that likely preceded the DNA world.[24] (Also see RNA world hypothesis.)

In Non-humans[edit]

See H5N1 for the current epizootic (an epidemic in nonhumans) and panzootic (a disease affecting animals of many species especially over a wide area) of H5N1 influenza
Avian influenza

Fowl act as natural asymptomatic carriers of influenza A viruses. Prior to the current H5N1 epizootic, strains of influenza A virus had been demonstrated to be transmitted from wild fowl to only birds, pigs, horses, seals, whales and humans; and only between humans and pigs and between humans and domestic fowl; and not other pathways such as domestic fowl to horse.[25]

Wild aquatic birds are the natural hosts for a large variety of influenza A viruses. Occasionally, viruses are transmitted from these birds to other species and may then cause devastating outbreaks in domestic poultry or give rise to human influenza pandemics.[2][3]

H5N1 has been shown to be transmitted to tigers, leopards, and domestic cats that were fed uncooked domestic fowl (chickens) with the virus. H3N8 viruses from horses have crossed over and caused outbreaks in dogs. Laboratory mice have been infected successfully with a variety of avian flu genotypes.[26]

Influenza A viruses spread in the air and in manure, and survives longer in cold weather. It can also be transmitted by contaminated feed, water, equipment and clothing; however, there is no evidence the virus can survive in well-cooked meat. Symptoms in animals vary, but virulent strains can cause death within a few days.

"Highly pathogenic avian influenza virus is on every top ten list available for potential agricultural bioweapon agents".[27]

Avian influenza viruses that the OIE and others test for to control poultry disease include: H5N1, H7N2, H1N7, H7N3, H13N6, H5N9, H11N6, H3N8, H9N2, H5N2, H4N8, H10N7, H2N2, H8N4, H14N5, H6N5, H12N5 and others.

Known outbreaks of highly pathogenic flu in poultry 1959–2003[28]
YearAreaAffectedSubtype
1959ScotlandChickenH5N1
1963EnglandTurkeyH7N3
1966Ontario (Canada)TurkeyH5N9
1976Victoria (Australia)ChickenH7N7
1979GermanyChickenH7N7
1979EnglandTurkeyH7N7
1983Pennsylvania (USA)*Chicken, turkeyH5N2
1983IrelandTurkeyH5N8
1985Victoria (Australia)ChickenH7N7
1991EnglandTurkeyH5N1
1992Victoria (Australia)ChickenH7N3
1994Queensland (Australia)ChickenH7N3
1994Mexico*ChickenH5N2
1994Pakistan*ChickenH7N3
1997New South Wales (Australia)ChickenH7N4
1997Hong Kong (China)*ChickenH5N1
1997ItalyChickenH5N2
1999Italy*TurkeyH7N1
2002Hong Kong (China)ChickenH5N1
2002ChileChickenH7N3
2003Netherlands*ChickenH7N7

*Outbreaks with significant spread to numerous farms, resulting in great economic losses. Most other outbreaks involved little or no spread from the initially infected farms.

1979: "More than 400 harbor seals, most of them immature, died along the New England coast between December 1979 and October 1980 of acute pneumonia associated with influenza virus, A/Seal/Mass/1/180 (H7N7)."[29]

1995: "[V]accinated birds can develop asymptomatic infections that allow virus to spread, mutate, and recombine (ProMED-mail, 2004j). Intensive surveillance is required to detect these “silent epidemics” in time to curtail them. In Mexico, for example, mass vaccination of chickens against epidemic H5N2 influenza in 1995 has had to continue in order to control a persistent and evolving virus (Lee et al., 2004)."[30]

1997: "Influenza A viruses normally seen in one species sometimes can cross over and cause illness in another species. For example, until 1997, only H1N1 viruses circulated widely in the U.S. pig population. However, in 1997, H3N2 viruses from humans were introduced into the pig population and caused widespread disease among pigs. Most recently, H3N8 viruses from horses have crossed over and caused outbreaks in dogs."[31]

2000: "In California, poultry producers kept their knowledge of a recent H6N2 avian influenza outbreak to themselves due to their fear of public rejection of poultry products; meanwhile, the disease spread across the western United States and has since become endemic."[32]

2003: In Netherlands H7N7 influenza virus infection broke out in poultry on several farms.[33]

2004: In North America, the presence of avian influenza strain H7N3 was confirmed at several poultry farms in British Columbia in February 2004. As of April 2004, 18 farms had been quarantined to halt the spread of the virus.[34]

2005: Tens of millions of birds died of H5N1 influenza and hundreds of millions of birds were culled to protect humans from H5N1. H5N1 is endemic in birds in southeast Asia and represents a long-term pandemic threat.

2006: H5N1 spreads across the globe, killing hundreds of millions of birds and over 100 people, and causing a significant H5N1 impact from both actual deaths and predicted possible deaths.

Swine flu
Swine influenza (or "pig influenza") refers to a subset of Orthomyxoviridae that create influenza and are endemic in pigs. The species of Orthomyxoviridae that can cause flu in pigs are influenza A virus and influenza C virus, but not all genotypes of these two species infect pigs. The known subtypes of influenza A virus that create influenza and are endemic in pigs are H1N1, H1N2, H3N1 and H3N2.
Horse flu
Horse flu (or "equine influenza") refers to varieties of influenza A virus that affect horses. Horse flu viruses were only isolated in 1956. The two main types of virus are called equine-1 (H7N7), which commonly affects horse heart muscle, and equine-2 (H3N8), which is usually more severe.
Dog flu
Dog flu (or "canine influenza") refers to varieties of influenza A virus that affect dogs. The equine influenza virus H3N8 was found to infect and kill – with respiratory illness – greyhound race dogs at a Florida racetrack in January 2004.
H3N8
H3N8 is now endemic in birds, horses and dogs.

Human influenza virus[edit]

"Human influenza virus" usually refers to those subtypes that spread widely among humans. H1N1, H1N2, and H3N2 are the only known influenza A virus subtypes currently circulating among humans.[35]

Genetic factors in distinguishing between "human flu viruses" and "avian influenza viruses" include:

PB2: (RNA polymerase): Amino acid (or residue) position 627 in the PB2 protein encoded by the PB2 RNA gene. Until H5N1, all known avian influenza viruses had a Glu at position 627, while all human influenza viruses had a lysine.
HA: (hemagglutinin): Avian influenza HA binds alpha 2–3 sialic acid receptors, while human influenza HA binds alpha 2–6 sialic acid receptors. Swine influenza viruses have the ability to bind both types of sialic acid receptors.

"About 52 key genetic changes distinguish avian influenza strains from those that spread easily among people, according to researchers in Taiwan, who analyzed the genes of more than 400 A type flu viruses."[36] "How many mutations would make an avian virus capable of infecting humans efficiently, or how many mutations would render an influenza virus a pandemic strain, is difficult to predict. We have examined sequences from the 1918 strain, which is the only pandemic influenza virus that could be entirely derived from avian strains. Of the 52 species-associated positions, 16 have residues typical for human strains; the others remained as avian signatures. The result supports the hypothesis that the 1918 pandemic virus is more closely related to the avian influenza A virus than are other human influenza viruses."[37]

Human flu symptoms usually include fever, cough, sore throat, muscle aches, conjunctivitis and, in severe cases, severe breathing problems and pneumonia that may be fatal. The severity of the infection will depend in large part on the state of the infected person's immune system and if the victim has been exposed to the strain before, and is therefore partially immune.

Highly pathogenic H5N1 avian influenza in a human is far worse, killing 50% of humans that catch it. In one case, a boy with H5N1 experienced diarrhea followed rapidly by a coma without developing respiratory or flu-like symptoms.[38]

The influenza A virus subtypes that have been confirmed in humans, ordered by the number of known human pandemic deaths, are:

H1N1
H1N1 is currently pandemic in both human and pig populations. A variant of H1N1 was responsible for the Spanish flu pandemic that killed some 50 million to 100 million people worldwide over about a year in 1918 and 1919.[39] Another variant was named a pandemic threat in the 2009 flu pandemic. Controversy arose in October, 2005, after the H1N1 genome was published in the journal, Science, because of fears that this information could be used for bioterrorism.[citation needed]
H2N2
The Asian flu, a pandemic outbreak of H2N2 avian influenza, originated in China in 1957, spread worldwide that same year during which a influenza vaccine was developed, lasted until 1958 and caused between one and four million deaths.
H3N2
H3N2 is currently endemic in both human and pig populations. It evolved from H2N2 by antigenic shift and caused the Hong Kong flu pandemic of 1968 and 1969 that killed up to 750,000.[40] "An early-onset, severe form of influenza A H3N2 made headlines when it claimed the lives of several children in the United States in late 2003."[41]
The dominant strain of annual flu in January 2006 was H3N2. Measured resistance to the standard antiviral drugs amantadine and rimantadine in H3N2 increased from 1% in 1994 to 12% in 2003 to 91% in 2005.[42]
"[C]ontemporary human H3N2 influenza viruses are now endemic in pigs in southern China and can reassort with avian H5N1 viruses in this intermediate host."[12]
H5N1
H5N1 is the world's major influenza pandemic threat.
"When he compared the 1918 virus with today's human flu viruses, Dr. Taubenberger noticed that it had alterations in just 25 to 30 of the virus's 4,400 amino acids. Those few changes turned a bird virus into a killer that could spread from person to person."[43]
H7N7
H7N7 has unusual zoonotic potential. In 2003 in Netherlands, 89 people were confirmed to have H7N7 influenza virus infection following an outbreak in poultry on several farms. One death was recorded.
H7N9
On 2 April 2013, the Centre for Health Protection (CHP) of the Department of Health of Hong Kong confirmed four more cases in Jiangsu province in addition to the three cases initially reported on 31 March 2013.[44]
H1N2
H1N2 is currently endemic in both human and pig populations. The new H1N2 strain appears to have resulted from the reassortment of the genes of the currently circulating influenza H1N1 and H3N2 subtypes. The hemagglutinin protein of the H1N2 virus is similar to that of the currently circulating H1N1 viruses, and the neuraminidase protein is similar to that of the current H3N2 viruses.
H9N2
Low pathogenic avian influenza A (H9N2) infection was confirmed in 1999, in China and Hong Kong in two children, and in 2003 in Hong Kong in one child. All three fully recovered.[45]
H7N2
One person in New York in 2003 and one person in Virginia in 2002 were found to have serologic evidence of infection with H7N2. Both fully recovered.[45]
H7N3
In North America, the presence of avian influenza strain H7N3 was confirmed at several poultry farms in British Columbia in February 2004. As of April 2004, 18 farms had been quarantined to halt the spread of the virus. Two cases of humans with avian influenza have been confirmed in that region. "Symptoms included conjunctivitis and mild influenza-like illness."[34] Both fully recovered.
H5N2
Japan's Health Ministry said January 2006 that poultry farm workers in Ibaraki prefecture may have been exposed to H5N2 in 2005.[46] The H5N2 antibody titers of paired sera of 13 subjects increased fourfold or more.[47]
H10N7
In 2004 in Egypt, H10N7 was reported for the first time in humans. It caused illness in two infants in Egypt. One child’s father is a poultry merchant.[48]

Evolution[edit]

Taubenberger says:

"All influenza A pandemics since [the Spanish flu pandemic], and indeed almost all cases of influenza A worldwide (excepting human infections from avian viruses such as H5N1 and H7N7), have been caused by descendants of the 1918 virus, including "drifted" H1N1 viruses and reassorted H2N2 and H3N2 viruses. The latter are composed of key genes from the 1918 virus, updated by subsequently incorporated avian influenza genes that code for novel surface proteins, making the 1918 virus indeed the "mother" of all pandemics."[49]

Researchers from the National Institutes of Health used data from the Influenza Genome Sequencing Project and concluded that during the ten-year period examined, most of the time the hemagglutinin gene in H3N2 showed no significant excess of mutations in the antigenic regions while an increasing variety of strains accumulated. This resulted in one of the variants eventually achieving higher fitness, becoming dominant, and in a brief interval of rapid evolution, rapidly sweeping through the population and eliminating most other variants.[50]

Short-term Evolution In the short-term evolution of influenza A virus, a 2006 study found that stochastic, or random, processes are key factors.[51] Influenza A virus HA antigenic evolution appears to be characterized more by punctuated, sporadic jumps as opposed to a constant rate of antigenic change.[52] Using phylogenetic analysis of 413 complete genomes of human influenza A viruses that were collected throughout the state of New York, the authors of Nelson et al. 2006 were able to show that genetic diversity, and not antigenic drift, shaped the short-term evolution of influenza A via random migration and reassortment. The evolution of these viruses is dominated more by the random importation of genetically different viral strains from other geographic locations and less by natural selection. Within a given season, adaptive evolution is infrequent and had an overall weak effect as evidenced from the data gathered from the 413 genomes. Phylogenetic analysis revealed the different strains were derived from newly imported genetic material as opposed to isolates that had been circulating in New York in previous seasons. Therefore, the gene flow in and out of this population, and not natural selection, was more important in the short term.

See also[edit]

Notes[edit]

  1. ^ "Avian influenza (" bird flu") — Fact sheet". WHO. 
  2. ^ a b Klenk, et al. (2008). "Avian Influenza: Molecular Mechanisms of Pathogenesis and Host Range". Animal Viruses: Molecular Biology. Caister Academic Press. ISBN 978-1-904455-22-6. 
  3. ^ a b Kawaoka Y, ed. (2006). Influenza Virology: Current Topics. Caister Academic Press. ISBN 1-904455-06-9. 
  4. ^ a b "Influenza Type A Viruses and Subtypes". Centers for Disease Control and Prevention. 2 April 2013. Retrieved 13 June 2013. 
  5. ^ "Unique new flu virus found in bats". NHS Choices. 1 March 2012. Retrieved 16 May 2012. 
  6. ^ Gewin, V. (2012). "Bats harbour influenza". Nature. doi:10.1038/nature.2012.10112.  edit
  7. ^ Tong, S.; Li, Y.; Rivailler, P.; Conrardy, C.; Castillo, D. A. A.; Chen, L. -M.; Recuenco, S.; Ellison, J. A.; Davis, C. T.; York, I. A.; Turmelle, A. S.; Moran, D.; Rogers, S.; Shi, M.; Tao, Y.; Weil, M. R.; Tang, K.; Rowe, L. A.; Sammons, S.; Xu, X.; Frace, M.; Lindblade, K. A.; Cox, N. J.; Anderson, L. J.; Rupprecht, C. E.; Donis, R. O. (2012). "A distinct lineage of influenza a virus from bats". Proceedings of the National Academy of Sciences. doi:10.1073/pnas.1116200109.  edit
  8. ^ Suxiang Tong et al. (October 2013). "New World Bats Harbor Diverse Influenza A Viruses". PLoS Pathogens. doi:10.1371/journal.ppat.1003657. 
  9. ^ Gallagher, James (29 July 2011). "'Super antibody' fights off flu". BBC News. Retrieved 29 July 2011. 
  10. ^ whitehouse.gov National Strategy for Pandemic Influenza — Introduction — "Although remarkable advances have been made in science and medicine during the past century, we are constantly reminded that we live in a universe of microbes – viruses, bacteria, protozoa and fungi that are forever changing and adapting themselves to the human host and the defenses that humans create. Influenza viruses are notable for their resilience and adaptability. While science has been able to develop highly effective vaccines and treatments for many infectious diseases that threaten public health, acquiring these tools is an ongoing challenge with the influenza virus. Changes in the genetic makeup of the virus require us to develop new vaccines on an annual basis and forecast which strains are likely to predominate. As a result, and despite annual vaccinations, the U.S. faces a burden of influenza that results in approximately 36,000 deaths and more than 200,000 hospitalizations each year. In addition to this human toll, influenza is annually responsible for a total cost of over $10 billion in the U.S. A pandemic, or worldwide outbreak of a new influenza virus, could dwarf this impact by overwhelming our health and medical capabilities, potentially resulting in hundreds of thousands of deaths, millions of hospitalizations, and hundreds of billions of dollars in direct and indirect costs. This Strategy will guide our preparedness and response activities to mitigate that impact."
  11. ^ Daum LT, Shaw MW, Klimov AI, et al. (August 2005). "Influenza A (H3N2) outbreak, Nepal". Emerging Infect. Dis. 11 (8): 1186–91. doi:10.3201/eid1108.050302. PMC 3320503. PMID 16102305. 
    "The 2003–2004 influenza season was severe in terms of its impact on illness because of widespread circulation of antigenically distinct influenza A (H3N2) Fujian-like viruses. These viruses first appeared late during the 2002–2003 influenza season and continued to persist as the dominant circulating strain throughout the subsequent 2003–2004 influenza season, replacing the A/Panama/2007/99-like H3N2 viruses (1). Of the 172 H3N2 viruses genetically characterized by the Department of Defense in 2003–2004, only one isolate (from Thailand) belonged to the A/Panama-like lineage. In February 2003, the World Health Organization (WHO) changed the H3N2 component for the 2004–2005 influenza vaccine to afford protection against the widespread emergence of Fujian-like viruses (2). The annually updated trivalent vaccine consists of hemagglutinin (HA) surface glycoprotein components from influenza H3N2, H1N1, and B viruses."
  12. ^ a b Mahmoud 2005, p. 126
    "H5N1 virus is now endemic in poultry in Asia (Table 2-1) and has gained an entrenched ecological niche from which to present a long-term pandemic threat to humans. At present, these viruses are poorly transmitted from poultry to humans, and there is no conclusive evidence of human-to-human transmission. However, continued, extensive exposure of the human population to H5N1 viruses increases the likelihood that the viruses will acquire the necessary characteristics for efficient human-to-human transmission through genetic mutation or reassortment with a prevailing human influenza A virus. Furthermore, contemporary human H3N2 influenza viruses are now endemic in pigs in southern China (Peiris et al., 2001) and can reassort with avian H5N1 viruses in this 'intermediate host.' Therefore, it is imperative that outbreaks of H5N1 disease in poultry in Asia are rapidly and sustainably controlled. The seasonality of the disease in poultry, together with the control measures already implemented, are likely to reduce temporarily the frequency of H5N1 influenza outbreaks and the probability of human infection."
  13. ^ BBC: 'Super antibody' fights off flu
  14. ^ Independent: Scientists hail the prospect of a universal vaccine for flu
  15. ^ Huffington Post: Universal Flu Vaccine On The Horizon: Researchers Find 'Super Antibody'
  16. ^ Clinical Services Journal article Avian influenza issues analysed published March 2006
  17. ^ MicrobiologyBytes: Virology: Orthomyxoviruses
  18. ^ UniProtKB/Swiss-Prot entry P09345 Complete sequence of a cDNA clone of the hemagglutinin gene of influenza A/Chicken/Scotland/59 (H5N1) virus: comparison with contemporary North American and European strains.
  19. ^ RNA Journal article Identification of potential conserved RNA secondary structure throughout influenza A coding regions published May 2011
  20. ^ BARRY RD (August 1961). "The multiplication of influenza virus. II. Multiplicity reactivation of ultraviolet irradiated virus". Virology 14: 398–405. PMID 13687359. 
  21. ^ HENLE W, LIU OC (October 1951). "Studies on host-virus interactions in the chick embryo-influenza virus system. VI. Evidence for multiplicity reactivation of inactivated virus". J. Exp. Med. 94 (4): 305–22. PMC 2136114. PMID 14888814. 
  22. ^ Gilker JC, Pavilanis V, Ghys R (June 1967). "Multiplicity reactivation in gamma irradiated influenza viruses". Nature 214 (5094): 1235–7. doi:10.1038/2141235a0. PMID 6066111. 
  23. ^ Peterhans E (May 1997). "Oxidants and antioxidants in viral diseases: disease mechanisms and metabolic regulation". J. Nutr. 127 (5 Suppl): 962S–965S. PMID 9164274. 
  24. ^ Bernstein H, Byerly HC, Hopf FA, Michod RE (October 1984). "Origin of sex". J. Theor. Biol. 110 (3): 323–51. doi:10.1016/S0022-5193(84)80178-2. PMID 6209512. 
  25. ^ Mahmoud 2005, p. 30
  26. ^ Mahmoud 2005, p. 82
    "Interestingly, recombinant influenza viruses containing the 1918 HA and NA and up to three additional genes derived from the 1918 virus (the other genes being derived from the A/WSN/33 virus) were all highly virulent in mice (Tumpey et al., 2004). Furthermore, expression microarray analysis performed on whole lung tissue of mice infected with the 1918 HA/ NA recombinant showed increased upregulation of genes involved in apoptosis, tissue injury, and oxidative damage (Kash et al., 2004). These findings were unusual because the viruses with the 1918 genes had not been adapted to mice. The completion of the sequence of the entire genome of the 1918 virus and the reconstruction and characterization of viruses with 1918 genes under appropriate biosafety conditions will shed more light on these findings and should allow a definitive examination of this explanation. Antigenic analysis of recombinant viruses possessing the 1918 HA and NA by hemagglutination inhibition tests using ferret and chicken antisera suggested a close relationship with the A/swine/Iowa/30 virus and H1N1 viruses isolated in the 1930s (Tumpey et al., 2004), further supporting data of Shope from the 1930s (Shope, 1936). Interestingly, when mice were immunized with different H1N1 virus strains, challenge studies using the 1918-like viruses revealed partial protection by this treatment, suggesting that current vaccination strategies are adequate against a 1918-like virus (Tumpey et al., 2004)."
  27. ^ Mahmoud 2005, p. 285
    "As of October 2001, the potential for use of infectious agents, such as anthrax, as weapons has been firmly established. It has been suggested that attacks on a nation’s agriculture might be a preferred form of terrorism or economic disruption that would not have the attendant stigma of infecting and causing disease in humans. Highly pathogenic avian influenza virus is on every top ten list available for potential agricultural bioweapon agents, generally following foot and mouth disease virus and Newcastle disease virus at or near the top of the list. Rapid detection techniques for bioweapon agents are a critical need for the first-responder community, on a par with vaccine and antiviral development in preventing spread of disease."
  28. ^ "Avian influenza A(H5N1)- update 31: Situation (poultry) in Asia: need for a long-term response, comparison with previous outbreaks". Epidemic and Pandemic Alert and Response (EPR). WHO. 2004. 
    Known outbreaks of highly pathogenic flu in poultry 1959–2003.
  29. ^ Geraci JR, St Aubin DJ, Barker IK, et al. (February 1982). "Mass mortality of harbor seals: pneumonia associated with influenza A virus". Science 215 (4536): 1129–31. doi:10.1126/science.7063847. PMID 7063847. "More than 400 harbor seals, most of them immature, died along the New England coast between December 1979 and October 1980 of acute pneumonia associated with influenza virus, A/Seal/Mass/1/180 (H7N7). The virus has avian characteristics, replicates principally in mammals, and causes mild respiratory disease in experimentally infected seals. Concurrent infection with a previously undescribed mycoplasma or adverse environmental conditions may have triggered the epizootic. The similarities between this epizootic and other seal mortalities in the past suggest that these events may be linked by common biological and environmental factors." 
  30. ^ Mahmoud 2005, p. 15
    "Unlike most other affected countries, Indonesia also instituted mass vaccination of healthy domestic birds against H5N1, followed by routine vaccination (China has a similar policy; other Asian countries are considering it [ProMED-mail, 2004j]) (Soebandrio, 2004). This is a risky strategy, because vaccinated birds can develop asymptomatic infections that allow virus to spread, mutate, and recombine (ProMED-mail, 2004j). Intensive surveillance is required to detect these "silent epidemics" in time to curtail them. In Mexico, for example, mass vaccination of chickens against epidemic H5N2 influenza in 1995 has had to continue in order to control a persistent and evolving virus (Lee et al., 2004)."
  31. ^ CDC Centers for Disease Control and Prevention — Transmission of Influenza A Viruses Between Animals and People
  32. ^ Mahmoud 2005, p. 27
  33. ^ BBC News Early bird flu warning for Dutch — 6 November 2005
  34. ^ a b Tweed SA, Skowronski DM, David ST, et al. (December 2004). "Human illness from avian influenza H7N3, British Columbia". Emerging Infect. Dis. 10 (12): 2196–9. doi:10.3201/eid1012.040961. PMC 3323407. PMID 15663860. 
  35. ^ CDC Key Facts About Avian Influenza (Bird Flu) and Avian Influenza A (H5N1) Virus
  36. ^ Bloomberg News article Scientists Move Closer to Understanding Flu Virus Evolution published 28 August 2006
  37. ^ Chen GW, Chang SC, Mok CK, et al. (September 2006). "Genomic signatures of human versus avian influenza A viruses". Emerging Infect. Dis. 12 (9): 1353–60. doi:10.3201/eid1209.060276. PMC 3294750. PMID 17073083. 
  38. ^ de Jong MD, Bach VC, Phan TQ, et al. (February 2005). "Fatal avian influenza A (H5N1) in a child presenting with diarrhea followed by coma". N. Engl. J. Med. 352 (7): 686–91. doi:10.1056/NEJMoa044307. PMID 15716562. 
  39. ^ Mahmoud 2005, p. 7
  40. ^ Detailed chart of its evolution here at PDF called Ecology and Evolution of the Flu
  41. ^ Mahmoud 2005, p. 115
    "There is particular pressure to recognize and heed the lessons of past influenza pandemics in the shadow of the worrisome 2003–2004 flu season. An early-onset, severe form of influenza A H3N2 made headlines when it claimed the lives of several children in the United States in late 2003. As a result, stronger than usual demand for annual flu inactivated vaccine outstripped the vaccine supply, of which 10 to 20 percent typically goes unused. Because statistics on pediatric flu deaths had not been collected previously, it is unknown if the 2003–2004 season witnessed a significant change in mortality patterns."
  42. ^ Reason New York Times This Season's Flu Virus Is Resistant to 2 Standard Drugs By Altman Published: 15 January 2006
  43. ^ New York Times Published: 8 November 2005 — Hazard in Hunt for New Flu: Looking for Bugs in All the Wrong Places
  44. ^ Schnirring, Lisa (2 April 2013). "China reports 4 more H7N9 infections". CIDRAP News. 
  45. ^ a b CDC Avian Influenza Infection in Humans
  46. ^ CBS News article Dozens In Japan May Have Mild Bird Flu January 2006.
  47. ^ Ogata T, Yamazaki Y, Okabe N, Nakamura Y, Tashiro M, et al. (July 2008). "Human H5N2 Avian Influenza Infection in Japan and the Factors Associated with High H5N2-Neutralizing Antibody Titer" (PDF). J Epidemiol 18 (4): 160–6. doi:10.2188/jea.JE2007446. PMID 18603824. 
  48. ^ niaid.nih.gov Timeline of Human Flu Pandemics
  49. ^ Taubenberger JK, Morens DM (January 2006). "1918 Influenza: the mother of all pandemics". Emerging Infect. Dis. 12 (1): 15–22. doi:10.3201/eid1201.050979. PMC 3291398. PMID 16494711. 
  50. ^ Science Daily article New Study Has Important Implications For Flu Surveillance published 27 October 2006
  51. ^ Nelson, Martha; L. Simonsen, C. Viboud, M. A. Miller, J. Taylor, K. S. George, S. B. Griesemer et al. (December 2006). "Stochastic Processes Are Key Determinants of Short-Term Evolution in Influenza A Virus". PLoS Pathogens. 
  52. ^ Smith, Derek J.; A. S. Lapedes, J. C. de Jong, T. M. Bestebroer, G. F. Rimmelzwaan, A. D. M. E. Osterhaus, and R. A. M. Fouchier. (July 2004). "Mapping the Antigenic and Genetic Evolution of Influenza Virus". Science 305 (5682): 371–376. doi:10.1126/science.1097211. PMID 15218094. Retrieved 14 November 2011. 

Further reading[edit]

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