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Ploidy is the number of sets of chromosomes in the nucleus of a cell. Normally a gamete (sperm or egg) carries a full set of chromosomes that includes a single copy of each chromosome, as aneuploidy generally leads to severe genetic disease in the offspring. The haploid number (n) is the number of chromosomes in a gamete. Two gametes form a diploid zygote with twice this number (2n) i.e. two copies of autosomal chromosomes. However, the sex chromosomes of diploid cells (excluding pseudoautosomal regions), which are subject to sex linkage, may be considered as haploid chromosomes, since haploid is also the term used to define a set of chromosomes with only one copy in the cell. For humans, a diploid species, x = n = 23. A typical human somatic cell contains 46 chromosomes: 2 complete haploid sets, which make up 23 homologous chromosome pairs.
Because chromosome number is generally reduced only by the specialized process of meiosis, the somatic cells of the body inherit and maintain the chromosome number of the zygote. However, in many situations somatic cells double their copy number by means of endoreduplication as an aspect of cellular differentiation. For example, the hearts of two-year-old children contain 85% diploid and 15% tetraploid nuclei, but by 12 years of age the proportions become approximately equal, and adults examined contained 27% diploid, 71% tetraploid and 2% octaploid nuclei.
Cells are described according to the number of sets present (the ploidy level): monoploid (1 set), diploid (2 sets), triploid (3 sets), tetraploid (4 sets), pentaploid (5 sets), hexaploid (6 sets), heptaploid or septaploid (7 sets), etc. The generic term polyploid is frequently used to describe cells with three or more sets of chromosomes (triploid or higher ploidy).
The term ploidy is a back-formation from haploid and diploid. These two terms are from Greek ἁπλόος haplóos "single" and διπλόος diplóos "double" combined with εἶδος eîdos "form" (compare idol from Latin īdōlum, that from Greek εἴδωλον eídōlon derived from εἶδος eîdos). The two haploid and diploid terms were borrowed from German through William Henry Lang's 1908 translation of an 1894 textbook by Eduard Strasburger and colleagues. Strasburger used diploid to refer to an organism with twice the number of chromosomes of a haploid organism, hence "double" and "single".
Technically, ploidy is a description of a nucleus. Though at times authors may report the total ploidy of all nuclei present within the cell membrane of a syncytium, usually the ploidy of the nuclei present will be described. For example, a fungal dikaryon with two haploid nuclei is distinguished from the diploid in which the chromosomes share a nucleus and can be shuffled together. Nonetheless, because in most situations there is only one nucleus, it is commonplace to speak of the ploidy of a cell.
Common wheat is an organism where x and n differ. It has six sets of chromosomes, two sets from each of three different diploid species that are its distant ancestors. The somatic cells are hexaploid, with six sets of chromosomes, 2n = 6x = 42. The gametes are haploid for their own species, but triploid, with three sets of chromosomes, by comparison to a probable evolutionary ancestor, einkorn wheat. The monoploid number x = 7, and the haploid number n = 21. Tetraploidy (four sets of chromosomes, 2n = 4x) is common in plants, and also occurs in amphibians, reptiles, and insects.
It is also possible on rare occasions for the ploidy to increase in the germline, which can result in polyploid offspring and ultimately polyploid species. This is an important evolutionary mechanism in both plants and animals. As a result, it becomes desirable to distinguish between the ploidy of a species or variety as it presently breeds and that of an ancestor. The number of chromosomes in the ancestral (non-homologous) set is called the monoploid number (x), and is distinct from the haploid number (n) in the organism as it now reproduces. Both numbers n, and x, apply to every cell of a given organism.
Over evolutionary time scales in which chromosomal polymorphisms accumulate, these changes become less apparent by karyotype - for example, humans are generally regarded as diploid, but the 2R hypothesis has confirmed two rounds of whole genome duplication in early vertebrate ancestors.
Ploidy can also differ with life cycle and in some insects it differs by caste. In humans, only the gametes are haploid, but in the Australian bulldog ant, Myrmecia pilosula, a haplodiploid species, haploid individuals of this species have a single chromosome, and diploid individuals have two chromosomes. In Entamoeba, the ploidy level varies of 4n to 40n in a single population. Alternation of generations occurs in many plants.
Some studies suggest that selection is more likely to favor diploidy in host species and haploidy in parasite species.
The nucleus of a eukaryotic cell is haploid if it has a single set of chromosomes, each one not being part of a pair. By extension a cell may be called haploid if its nucleus is haploid, and an organism may be called haploid if its body cells (somatic cells) are haploid. The number of chromosomes in a single set is called the haploid number, given the symbol n.
Gametes (sperm and ova) are haploid cells. The haploid gametes produced by most organisms combine to form a zygote with n pairs of chromosomes, one from each gamete, i.e. 2n chromosomes in total. The chromosomes in each pair, one of which comes from the sperm and one from the egg, are said to be homologous. Cells and organisms with pairs of homologous chromosomes are called diploid. For example, most animals are diploid and produce haploid gametes. During meiosis, sex cell precursors have their number of chromosomes halved by randomly "choosing" one member of each pair of chromosomes, resulting in haploid gametes. Because homologous chromosomes usually differ genetically, gametes usually differ genetically from one another.
All plants and many fungi and algae switch between a haploid and a diploid state, with one of the stages emphasized over the other. This is called alternation of generations. Most fungi and algae are haploid during the principal stage of their lifecycle, as are plants like mosses. Most animals are diploid, but male bees, wasps, and ants are haploid organisms because they develop from unfertilized, haploid eggs.
In some cases there is evidence that the n chromosomes in a haploid set have resulted from duplications of an originally smaller set of chromosomes. This "base" number – the number of apparently originally unique chromosomes in a haploid set – is called the monoploid number. As an example, the chromosomes of common wheat are believed to be derived from three different ancestral species, each of which had 7 chromosomes in its haploid gametes. The monoploid number is thus 7 and the haploid number is 3 × 7 = 21. In general n is a multiple of x. The somatic cells in a wheat plant have six sets of 7 chromosomes: three sets from the egg and three sets from the sperm which fused to form the plant, giving a total of 42 chromosomes. As a formula, for wheat 2n = 6x = 42, so that the haploid number n is 21 and the monoploid number x is 7. The gametes of common wheat are considered to be haploid, since they contain half the genetic information of somatic cells, but they are not monoploid, as they still contain three complete sets of chromosomes (n = 3x).
In the case of wheat, the origin of its haploid number of 21 chromosomes from three sets of 7 chromosomes can be demonstrated. In many other organisms, although the number of chromosomes may have originated in this way, this is no longer clear, and the monoploid number is regarded as the same as the haploid number. Thus in humans, x = n = 23.
Diploid cells have two homologous copies of each chromosome, usually one from the mother and one from the father. Nearly all mammals are diploid organisms (the tetraploid (four sets) plains viscacha rats Tympanoctomys barrerae and Pipanacoctomys aureus are the only known exceptions as of 2004), although all individuals have some small fraction of cells that display polyploidy. Human diploid cells have 46 chromosomes and human haploid gametes (egg and sperm) have 23 chromosomes. Retroviruses that contain two copies of their RNA genome in each viral particle are also said to be diploid. Examples include human foamy virus, human T-lymphotropic virus, and HIV.
"Homoploid" means "at the same ploidy level", i.e. having the same number of homologous chromosomes. For example, homoploid hybridization is hybridization where the offspring have the same ploidy level as the two parental species. This contrasts with a common situation in plants where chromosome doubling accompanies, or happens soon after hybridization. Similarly, homoploid speciation contrasts with polyploid speciation.
Zygoidy is the state where the chromosomes are paired and can undergo meiosis. The zygoid state of a species may be diploid or polyploid. In the azygoid state the chromosomes are unpaired. It may be the natural state of some asexual species or may occur after meiosis. In diploid organisms the azygoid state is monoploid. (see below for dihaploidy)
Polyploidy is the state where all cells have multiple sets of chromosomes beyond the basic set, usually 3 or more. Specific terms are triploid (3 sets), tetraploid (4 sets), pentaploid (5 sets), hexaploid (6 sets), heptaploid or septaploid (7 sets) octoploid (8 sets), nonaploid (9 sets), decaploid (10 sets), undecaploid (11 sets), dodecaploid (12 sets), tridecaploid (13 sets), tetradecaploid (14 sets) etc. Some higher ploidies include hexadecaploid, dotriacontaploid, and tetrahexacontaploid, though Greek terminology may be set aside for readability in cases of higher ploidy (such as "16-ploid"). Polytene chromosomes of plants and fruit flies can be 1024-ploid. Ploidy of systems such as the salivary gland, elaiosome, endosperm, and trophoblast can exceed this, up to 1048576-ploid in the silk glands of the commercial silkworm Bombyx mori.
The chromosome sets may be from the same species or from closely related species. In the latter case, these are known as allopolyploids (or amphidiploids, which are allopolyploids that behave as if they were normal diploids). Allopolyploids are formed from the hybridization of two separate species. In plants, this probably most often occurs from the pairing of meiotically unreduced gametes, and not by diploid–diploid hybridization followed by chromosome doubling. The so-called Brassica triangle is an example of allopolyploidy, where three different parent species have hybridized in all possible pair combinations to produce three new species.
Polyploidy occurs commonly in plants, but rarely in animals. Even in diploid organisms, many somatic cells are polyploid due to a process called endoreduplication where duplication of the genome occurs without mitosis (cell division).
The extreme in polyploidy occurs in the fern genus Ophioglossum, the adder's-tongues, in which polyploidy results in chromosome counts in the hundreds, or, in at least one case, well over one thousand.
It is also possible for polyploid organisms to revert to lower ploidy by means of haploidisation.
Depending on growth conditions, prokaryotes such as bacteria may have a chromosome copy number of 1 to 4, and that number is commonly fractional, counting portions of the chromosome partly replicated at a given time. This is because under exponential growth conditions the cells are able to replicate their DNA faster than they can divide.
Mixoploidy refers to the presence of two cell lines, one diploid and one polyploid. Though polyploidy in humans is not viable, mixoploidy has been found in live adults and children. There are two types: diploid-triploid mixoploidy, in which some cells have 46 chromosomes and some have 69, and diploid-tetraploid mixoploidy, in which some cells have 46 and some have 92 chromosomes.
Dihaploid and polyhaploid cells are formed by haploidisation of polyploids, i.e., by halving the chromosome constitution.
Dihaploids (which are diploid) are important for selective breeding of tetraploid crop plants (notably potatoes), because selection is faster with diploids than with tetraploids. Tetraploids can be reconstituted from the diploids, for example by somatic fusion.
The term “dihaploid” was coined by Bender to combine in one word the number of genome copies (diploid) and their origin (haploid). The term is well established in this original sense, but it has also been used for doubled monoploids or doubled haploids, which are homozygous and used for genetic research.
Euploidy is the state of a cell or organism having an integral multiple of the monoploid number, possibly excluding the sex-determining chromosomes. For example, a human cell has 46 chromosomes, which is an integer multiple of the monoploid number, 23. A human with abnormal, but integral, multiples of this full set (e.g. 69 chromosomes) would also be considered as euploid. Aneuploidy is the state of not having euploidy. In humans, examples include having a single extra chromosome (such as Down syndrome), or missing a chromosome (such as Turner syndrome). Aneuploid karyotypes are given names with the suffix -somy (rather than -ploidy, used for euploid karyotypes), such as trisomy and monosomy.
A study comparing the karyotypes of endangered or invasive plants with those of their relatives found that being polyploid as opposed to diploid is associated with a 14% lower risk of being endangered, and a 20% greater chance of being invasive. Polyploidy may be associated with increased vigor and adaptability.
Some eukaryotic genome-scale or genome size databases and other sources which may contain the ploidy of many organisms: