Evans blue

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Evans blue
Evans Blue.svg
Identifiers
CAS number314-13-6 YesY
PubChem6321418
KEGGC19422 N
MeSHEvans+blue
ChEMBLCHEMBL1200712 N
Jmol-3D imagesImage 1
Properties
Molecular formulaC34H24N6Na4O14S4
Molar mass960.81 g mol−1
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
 N (verify) (what is: YesY/N?)
Infobox references
 
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For the Canadian rock band, see Evans Blue.
Evans blue
Evans Blue.svg
Identifiers
CAS number314-13-6 YesY
PubChem6321418
KEGGC19422 N
MeSHEvans+blue
ChEMBLCHEMBL1200712 N
Jmol-3D imagesImage 1
Properties
Molecular formulaC34H24N6Na4O14S4
Molar mass960.81 g mol−1
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
 N (verify) (what is: YesY/N?)
Infobox references

T-1824 or Evans Blue, often incorrectly rendered as Evan's Blue, is an azo dye which has a very high affinity for serum albumin. Because of this, it can be useful in physiology in estimating the proportion of body water contained in blood plasma.[1]

Evans blue dye has been used as a viability assay on the basis of its penetration into non-viable cells, although the method is subject to error because damaged cells may be capable of repair[2]

Evans blue is also used to assess the permeability of the blood–brain barrier to macromolecules. Because serum albumin cannot cross the barrier, and virtually all Evans Blue is bound to albumin, normally the neural tissue remains unstained.[3] When the BBB has been compromised, albumin-bound Evans blue enters the CNS. It fluoresces with excitation peaks at 470 and 540 nm and an emission peak at 680 nm. [4]

It was named after Herbert McLean Evans, an American chemist.

References[edit]

  1. ^ Physiology at MCG 7/7ch02/7ch02p17
  2. ^ Crutchfield A, Diller K, Brand J (1999). "Cryopreservation of Chlamydomonas reinhardtii (Chloropkhyta)" (HTML). European Journal of Phycology 34 (1): 43–52. doi:10.1080/09670269910001736072. 
  3. ^ Hawkins BT, Egleton RD (2006). "Fluorescence imaging of blood–brain barrier disruption". Journal of Neuroscience Methods 151 (2): 262–7. doi:10.1016/j.jneumeth.2005.08.006. PMID 16181683. 
  4. ^ Hed J, Dahlgren C, and Rundquist, I (1983). "A Simple Fluorescence Technique to Stain the Plasma Membrane of Human Neutrophils". Histochemistry 79 (1): 105–10. doi:10.1007/BF00494347. PMID 6196326. 

External links[edit]