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Marley was born in Bangor, Maine. He then lived in the Munjoy Hill area of Portland and graduated from Deering High School in 1985. Marley went on to attend the University of Maine at Farmington, graduating with a degree in Community Health. However, even when in college, he knew that he wanted to pursue comedy as his career.
Marley's comedic bits are mostly about life in Maine. Bob Marley's first television appearance was on Comedy Central. He also appeared as Detective Greenly in the movie The Boondock Saints, and reprised his role as Detective Greenly in The Boondock Saints II: All Saints Day.
He is regularly hosted on Sirius Satellite Radio channels 97 Blue collar radio and 99 RawDog comedy and appears every week on Coast 93.1-WMGX in Portland, Maine, where his segment is called "The World According to Bob."
On September 23, 2010 at 11:02 PM Bob Marley entered the Guinness Book of World Records with the longest continuous standup routine. He completed 40 hours of standup comedy, the first 18 without any repetition of material.