Belimumab

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Belimumab ?
Monoclonal antibody
TypeWhole antibody
SourceHuman
TargetB-cell activating factor (BAFF, BLyS)
Clinical data
Trade namesBenlysta
AHFS/Drugs.comConsumer Drug Information
MedlinePlusa611027
Licence dataUS FDA:link
Pregnancy cat.C (US)
Legal status-only (US)
RoutesIntravenous
Identifiers
CAS number356547-88-1 N
ATC codeL04AA26
UNII73B0K5S26A YesY
KEGGD03068 N
Chemical data
FormulaC6714H10428O2102S52 
Mol. mass151.8 kDa
 N (what is this?)  (verify)
 
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Belimumab ?
Monoclonal antibody
TypeWhole antibody
SourceHuman
TargetB-cell activating factor (BAFF, BLyS)
Clinical data
Trade namesBenlysta
AHFS/Drugs.comConsumer Drug Information
MedlinePlusa611027
Licence dataUS FDA:link
Pregnancy cat.C (US)
Legal status-only (US)
RoutesIntravenous
Identifiers
CAS number356547-88-1 N
ATC codeL04AA26
UNII73B0K5S26A YesY
KEGGD03068 N
Chemical data
FormulaC6714H10428O2102S52 
Mol. mass151.8 kDa
 N (what is this?)  (verify)

Belimumab (trade name Benlysta, previously known as LymphoStat-B) is a human monoclonal antibody that inhibits B-cell activating factor (BAFF),[1] also known as B-lymphocyte stimulator (BLyS).[2] B cells are responsible for part of the normal immune response, and also for the over-aggressive immune response in autoimmune diseases like systemic lupus erythematosus (SLE).

Belimumab is approved in the United States, Canada and Europe for treatment of SLE. However, the major phase III trials excluded the more severe cases of SLE with kidney and brain damage, so its effectiveness has not been demonstrated in those cases. A Phase III study for SLE patients with kidney disease is now recruiting.[3]

U.S. F.D.A. reviewers were concerned that belimumab is only "marginally" effective, and that there were more deaths in the treatment group.

Belimumab's defenders said that in addition to its modest efficiency, belimumab allowed patients to significantly reduce their use of corticosteroids.[4]

Belimumab is expensive, typically $28,000 for the first year. The UK National Institute for Health and Care Excellence (NICE) calculated the cost of belimumab at £61,200 per quality adjusted life year (QALY), which is more than the normally accepted £20,000 to £30,000.

Phase II trials of belimumab for rheumatoid arthritis were unsuccessful. Phase II trials for Sjögren’s Syndrome were more successful.

Belimumab was developed by Human Genome Sciences (HGS) and Cambridge Antibody Technology.[5] GlaxoSmithKline acquired HGS, took belimumab through Phase III clinical trials, and markets belimumab.[6]

Uses[edit]

Systemic lupus erythematosus[edit]

While belimumab appears safe in systemic lupus erythematosus, the magnitude of benefit is small.[7] Black/African American patients did not show a benefit. The most severe cases, with kidney and central nervous system involvement, were excluded from the trials.

The efficacy and safety of belimumab was demonstrated in 2 Phase III randomized, controlled studies, BLISS-52[8] and BLISS-76.[9] The 2 studies had a total of 1,684 patients, with scores of ≥6 on the SELENA-SLEDAI assessment. They were divided into a placebo and 2 dosage groups of belimumab, in addition to standard therapy. The primary end point was a reduction of ≥4 on the SELENA-SLEDAI assessment, and several other factors, at 52 weeks. Belimumab significantly improved the response rate, reduced disease activity and severe flares, and was well tolerated. 58% had SELENA-SLEDAI scores reduced by ≥4 points during 52 weeks with belimumab 10 mg/kg compared to 46% with placebo.

Benlysta was the first new drug to treat lupus after 56 years. Sales rose to $31.2 million in the first quarter of 2012.[10] It is marketed by GlaxoSmithKline and sold for about US$35,000 per year per patient.

Rheumatoid arthritis[edit]

In phase II clinical trials for rheumatoid arthritis.[11] belimumab was not effective.[7]

Sjögren’s Syndrome[edit]

Belimumab has completed phase II trials for Sjögren’s Syndrome.[12]

Side effects[edit]

Common adverse effects reported with belimumab include nausea, diarrhea, fever, as well as hypersensitivity and infusion-site reactions (severe in 0.9% of patients). It is suggested that patients be treated with an antihistamine prior to a belimumab infusion.[13]

A greater number of serious infections and deaths were reported in patients treated with belimumab than in those treated with placebo. Infections are due to the immunosuppressant properties of the drug.[14][dead link]

Interactions[edit]

No interaction studies have been carried out. Combination of belimumab with other immunosuppressants, especially those targeting B lymphocytes such as anti-CD20 therapies, could increase the risk of severe infections. Likewise, the combination with cyclophosphamide is not recommended, as well as administering live vaccines during treatment with belimumab.[13][15]

Mechanism of action[edit]

B lymphocytes (B cells) are one of the immune cells responsible for the damage in autoimmune disease. B cells develop in the bone marrow and continue to mature peripherally in secondary lymphoid organs and (as recently discovered) in the gut. When autoimmune B cells attack the body's own tissues, they are normally destroyed by cell suicide (apoptosis). In order to survive, B cells need survival factors. Researchers theorize that SLE is caused when autoimmune B cells proliferate, and survival factors protect them from cell suicide.

B-cell activating factor (BAFF), also called B-lymphocyte stimulator (BLyS), is required for the development and survival of B cells. In SLE, BAFF is overexpressed. Researchers theorize that BAFF overexpression causes autoimmune B cell proliferation and survival, which causes SLE.

Belimumab is a human antibody that binds to BAFF, preventing BAFF from binding to B cells. Without the survival factor BAFF, B cells commit suicide, and no longer contribute to the autoimmune damage of SLE.

BAFF is secreted by a variety of cells: monocytes and macrophages, bone marrow stromal cells, astrocytes, synoviocytes during rheumatoid arthritis, salivary epithelial cells during Sjögren's syndrome, astrocytes in certain glioblastomas.

BAFF interacts with three membrane receptors on B lymphocytes:

When BAFF binds to BAFF-R and BCMA on B cells, levels of Bcl-2, a survival factor, are increased.

When all three BAFF receptors are stimulated, levels of NF kappa B, which contributes to cell proliferation and differentiation, are increased in the nucleus.

Another B-cell activator like BAFF is APRIL (a proliferation-inducing ligand),[16] but APRIL only activates BCMA and TACI, not BAFF-R.

Belimumab reduces the number of circulating B cells, but anti-CD20 monoclonal antibodies reduce the number even more. It is possible that belimumab binds primarily to circulating soluble BAFF, therefore not inducing antibody-dependent cellular cytotoxicity that could be expected from this IgG1-type antibody.[17][Better, more current citation needed]

Discovery and history[edit]

B-cell activating factor is a naturally occurring protein that was discovered by researchers from National Jewish Health (previously the National Jewish Medical and Research Center) and the University of Colorado, who collaboratively published a paper detailing their findings in May 1999, naming the protein TALL-1.[18] The same protein was named BAFF in another paper published in June 1999; and in a paper published in July of that year, Human Genome Sciences (HGS) referred to it as BLyS (or B lymphocyte stimulator).[19] Six years later, the key role of BLyS in B cell differentiation, survival and activation was published.[20]

Five years prior, in October 2000, HGS and Cambridge Antibody Technology (CAT) agreed to co-develop monoclonal antibodies targeted at BLyS. Under this agreement, CAT would identify antibodies and HGS would select appropriate ones to take into clinical trials.[21] In 2003, CAT researchers reported that, by using phage display technology, they had elicited an array of over 1000 distinct antibodies, half of which inhibited binding of BLyS to its receptor.[5] Later that year, one of these antibodies was isolated and characterized. It was named LymphoStat-B and subsequently called belimumab.[22]

In August 2006, HGS and GlaxoSmithKline (GSK) entered into a co-development and commercialization agreement under which HGS would conduct Phase 3 trials for belimumab with assistance from GSK. The companies would share equally in Phase 3/4 development costs, sales and marketing expenses, and profits of any product commercialized under the agreement.[21] On February 13, 2007, HGS and GSK announced the initiation of the first of two pivotal Phase 3 clinical trial of belimumab in patients with active lupus erythematosus.[6]

FDA approval[edit]

Under its trade name Benlysta, belimumab was approved by the U.S. Food and Drug Administration (FDA) for treatment of SLE on March 9, 2011.[23] The FDA Advisory committee approved it with a 13-to-2 vote, despite reservations that the improvement of only 4 points on the SELENA-SLEDA scale was only marginally effective, and despite reservations about additional deaths in the treatment group.[24][25] Based on the number needed to treat, approximately eleven patients must be treated for one to benefit. It was not tested in severe forms of SLE, which involve active damage to the kidneys or central nervous system in the phase III trials. Subjects with active kidney disease were included in the Phase II trials of the drug.[26] "Patients with active lupus that involved the kidneys ... were excluded from participating in the trials. Study participants of African American or African descent did not significantly respond to belimumab."[24][27][28] It has subsequently been approved for use in Europe and Canada.[29]

Costs[edit]

Belimumab is expensive. At a typical U.S. academic center, the total cost for the first year of treatment is $28,000. Annual prices of generic prescription drugs are prednisone, $140; hydroxychloroquine, $132; oral methotrexate, $432; azathioprine, $468; and mycophenolate mofetil, $1,224.[30] In the UK, the National Institute for Health and Care Excellence (NICE) calculated the cost of belimumab at £61,200 per quality adjusted life year (QALY). This is more than the normally accepted cost of £20,000 to £30,000 per QUALY. The manufacturer offered the UK National Health Service a discount, the amount of which were confidential, which still did not bring it into the acceptable range.[31]

Other drugs addressing B lymphocyte hyperactivity[edit]

Atacicept is a recombinant fusion protein built with the extracellular ligand binding portion of TACI. It blocks activation of TACI by APRIL and BLyS. It failed a phase II trial for multiple sclerosis.[32]

Blisibimod, an inhibitor of both soluble and membrane bound BAFF, has demonstrated similar reductions of B cells in clinical trials and is being investigated in a phase 2 clinical study for patients with lupus erythematosus.

BR3-Fc is a recombinant fusion protein built with the extracellular ligand-binding portion of BAFF-R. It blocks activation of this receptor by BLyS, and is in early stage pharmaceutical development.[33]

Of anti-CD20 monoclonal antibodies, rituximab has been approved for some indications. Ocrelizumab, ofatumumab and "third generation" anti-CD20 monoclonals are in development.

References[edit]

  1. ^ Bossen, C; Schneider, P (October 2006). "BAFF, APRIL and their receptors: structure, function and signaling". Semin. Immunol. 18 (5): 263–75. doi:10.1016/j.smim.2006.04.006. PMID 16914324. 
  2. ^ Srini V. Kaveri, Luc Mouthon, and Jagadeesh Bayry (September 9, 2010). "Clinical Implications of Basic Research: Basophils and Nephritis in Lupus". N Engl J Med 363 (11): 1080–1082. doi:10.1056/NEJMcibr1006936. PMID 20825323. 
  3. ^ Efficacy and Safety of Belimumab in Patients With Active Lupus Nephritis (BLISS-LN), NCT01639339
  4. ^ Should Belimumab Have Been Approved? Stephen Paget, Medscape Rheumatology, Mar 24, 2011
  5. ^ a b Edwards BM, Barash SC, Main SH, et al. (November 2003). "The remarkable flexibility of the human antibody repertoire; isolation of over one thousand different antibodies to a single protein, BLyS". J. Mol. Biol. 334 (1): 103–18. doi:10.1016/j.jmb.2003.09.054. PMID 14596803. 
  6. ^ a b "Human Genome Sciences And Glaxosmithkline Announce Initiation Of Phase 3 Clinical Trial Of Lymphostat-B In Systemic Lupus Erythematosus" (Press release). Human Genome Sciences. 2007-02-13. Archived from the original on 25 April 2011. Retrieved 2011-03-11. 
  7. ^ a b Belimumab: The 1st drug to be FDA approved for the treatment of lupus since 1955, By Rebecca Manno, Johns Hopkins Arthritis Center, July 15, 2011
  8. ^ Navarra SV, Guzmán RM, Gallacher et al., BLISS-52 Study Group. (Feb 26, 2011). "Efficacy and safety of belimumab in patients with active systemic lupus erythematosus: a randomised, placebo-controlled, phase 3 trial". Lancet. 377 (9767): 721–31. doi:10.1016/S0140-6736(10)61354-2. PMID 21296403. 
  9. ^ Furie R, Petri M, Zamani O, et al., BLISS-76 Study Group. (December 2011). "A phase III, randomized, placebo-controlled study of belimumab, a monoclonal antibody that inhibits B lymphocyte stimulator, in patients with systemic lupus erythematosus". Arthritis Rheum. 63 (12): 3918–30. doi:10.1002/art.30613. PMID 22127708. 
  10. ^ HGSI Cuts Loss on Benlysta Sales, By Zacks Equity Research, Apr 26, 2012
  11. ^ ClinicalTrials.gov NCT00071812 A Safety and Efficacy Study of LymphoStat-B (Monoclonal Anti-BLyS Antibody) in Subjects With Rheumatoid Arthritis,
  12. ^ Efficacy and Safety of Belimumab in Subjects With Primary Sjögren's Syndrome (BELISS)
  13. ^ a b European Medicines Agency: Benlysta Summary of Product Characteritics
  14. ^ "GlaxoSmithKline and Human Genome Sciences announce FDA approval of Benlysta (belimumab) for the treatment of systemic lupus erythematosus". GlaxoSmithKline. 9 March 2011. Retrieved 11 Mars 2011. 
  15. ^ Drugs.com: Benlysta Official FDA information, side effects and uses
  16. ^ Schneider P (2005). "The role of APRIL and BAFF in lymphocyte activation". Curr. Opin. Immunol. 17 (3): 282–9. doi:10.1016/j.coi.2005.04.005. PMID 15886118. 
  17. ^ June 2007 European League against Rheumatism symposium.
  18. ^ Shu HB, Hu WH, Johnson H (May 1999). "TALL-1 is a novel member of the TNF family that is down-regulated by mitogens". J. Leukoc. Biol. 65 (5): 680–3. PMID 10331498. 
  19. ^ Moore, PA; Belvedere, O; Orr, A; Pieri, K; Lafleur, DW; Feng, P; Soppet, D; Charters, M; Gentz, R; Parmelee, D; Li, Y; Galperina, O; Giri, J; Roschke, V; Nardelli, B; Carrell, J; Sosnovtseva, S; Greenfield, W; Ruben, SM; Olsen, HS; Fikes, J; Hilbert, DM (July 1999). "BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator". Science 285 (5425): 260–3. doi:10.1126/science.285.5425.260. PMID 10398604. 
  20. ^ Crowley JE, Treml LS, Stadanlick JE, Carpenter E, Cancro MP (2005). "Homeostatic niche specification among naïve and activated B cells: a growing role for the BLyS family of receptors and ligands". Semin. Immunol. 17 (3): 193–9. doi:10.1016/j.smim.2005.02.001. PMID 15826824. 
  21. ^ a b "Benlysta (belimumab)". Human Genome Sciences. Archived from the original on 21 April 2011. Retrieved 2011-03-11. 
  22. ^ Baker KP, Edwards BM, Main SH, et al. (November 2003). "Generation and characterization of LymphoStat-B, a human monoclonal antibody that antagonizes the bioactivities of B lymphocyte stimulator". Arthritis Rheum. 48 (11): 3253–65. doi:10.1002/art.11299. PMID 14613291. 
  23. ^ "FDA approves Benlysta to treat lupus" (Press release). U.S. Food and Drug Administration (FDA). March 9, 2011. 
  24. ^ a b FDA Questions Safety, Efficacy of Belimumab, By Emily P. Walker, Washington Correspondent, MedPage Today, November 12, 2010
  25. ^ F.D.A. Panel Backs Drug for Lupus By ANDREW POLLACK, New York Times, November 16, 2010M
  26. ^ Andrew Pollack, "F.D.A. Approves Benlysta, a New Lupus Drug", The New York Times, March 9, 2011
  27. ^ Summary Minutes of the Arthritis Advisory Committee Meeting November 16, 2010 U.S. Food and Drug Administration (FDA)
  28. ^ 2010 Meeting Materials, Arthritis Advisory Committee U.S. Food and Drug Administration (FDA)
  29. ^ WRAL Tech Wire: GSK wins OK for Lupus drug in Europe, Canada
  30. ^ Bevra Hannahs Hahn, M.D. (April 18, 2013). "Belimumab for Systemic Lupus Erythematosus". N Engl J Med 368 (16): 1528–1535. doi:10.1056/NEJMct1207259. PMID 23594005. 
  31. ^ NICE publishes draft guidance on belimumab for systemic lupus erythematosus, press release, 26 April 2012
  32. ^ ClinicalTrials.gov NCT00642902 Atacicept in Multiple Sclerosis, Phase II
  33. ^ Vugmeyster, Y.; Seshasayee, D.; Chang, W.; Storn, A.; Howell, K.; Sa, S.; Nelson, T.; Martin, F.; Grewal, I.; Gilkerson, E.; Wu, B.; Thompson, J.; Ehrenfels, B. N.; Ren, S.; Song, A.; Gelzleichter, T. R.; Danilenko, D. M. (2006). "A Soluble BAFF Antagonist, BR3-Fc, Decreases Peripheral Blood B Cells and Lymphoid Tissue Marginal Zone and Follicular B Cells in Cynomolgus Monkeys". The American Journal of Pathology 168 (2): 476–489. doi:10.2353/ajpath.2006.050600. PMC 1606502. PMID 16436662.  edit