The show starred Gail Davis in the title role, and co-starred Brad Johnson as Deputy Sheriff Lofty Craig and Jimmy Hawkins, as Annie's brother, Tagg. In one episode, "Bull's Eye", the role of Tagg was played by Billy Gray, better known as James "Bud" Anderson, Jr., on Father Knows Best. In the series, Annie Oakley rode a horse named Target, Tagg's horse was Pixie and Lofty's was named Forest. Annie and Tagg lived in the town of Diablo, Arizona, with their uncle, Sheriff Luke MacTavish, who was usually away whenever trouble started. It would then be up to straight-shooting Annie and her "silent suitor" Lofty Craig to rescue law-abiding neighbors and arrest outlaws. Often Tagg would be told to stay in town and out of the way, but through disobedience, the need to relay important new information, or being captured by outlaws, he would end up in the middle of the adventure.
Annie always wore the same fringed cowgirl outfit, of which 15 or more copies were made throughout the show's production. Her hairstyle was braided pigtails.
Additional episodes were being considered, but Jimmy Hawkins had a growth spurt and outgrew the part of Annie's little brother.
Alan Hale, Jr., as Eli Gorham in "Annie Calls Her Shots" and as Moose Bosco in "Annie and the Silver Ace" (both 1954)
Dick Jones, as Corporal Sam in "Annie Joins the Cavalry", as Bob Neil in "Annie Helps a Drifter", and as Steve Donavan in "Annie and the Six o'Spades" (all 1954) and as Clell Morgan in "Annie and the Junior Pioneers" (1955)
L. Q. Jones portrayed Cal Upton in "The Robin Hood Kid" and Ned Blane in "Dilemma at Diablo" (both 1956)
Douglas Kennedy appeared as Jim Hayward in "Annie Takes a Chance" and as Ralph Putnam in "Powder Rock Stampede" (both 1955).
Keye Luke appeared as Li Wong, a Chinese laundryman and landowner, in the 1955 episode "Annie and the Chinese Puzzle", which touches on racial discrimination.
Tyler MacDuff appeared in four episodes in 1956, including the title guest-starring role in "The Saga of Clement O'Toole" and as Don "Doc" Briggs in "Dilemma at Diablo".
Tyler McVey appeared in the 1956 episode "Showdown at Diablo" in the role of Senator Ridgeway.
Ewing Mitchell, as Ab Forsyth in "Annie and the Twisted Trails" and Major Paley in "Santa Claus Wears a Gun" (both 1956) and Colonel Granger in "Dude's Decision" (1957)
Fess Parker, as newspaper publisher Tom Conrad in "Annie and the Mystery Woman" and as Les Clinton in "Annie and the Texas Sandman" (both 1954)
William Phipps, as George Wessel in "Sure Shot Annie (1955), as Dan Carter in "Annie and the Twisted Trails" (1956), and as Earl Wallace in "Dude's Decision (1957)
Slim Pickens, as Sundown in "Annie and the Leprechauns", as Slim in "The Waco Kid", as Ed Morgan in "Annie Rings the Bell", and as Garner in "Grubstake Bank" (all 1956)
Denver Pyle, as Dr. Barnes in "Valley of the Shadows" and as Tom Malloy in "Annie's Desert Adventure" (both 1954)
Mike Ragan, four episodes, "The Runaways" (1954), "Thunder Hill" as henchman Walt Newman (1955), "Annie Rides the Navajo Trail" as Sgt. Yorkton (1956), and "Amateur Outlaw" (1956)
Arthur Space, as Carl Bishop in "Valley of the Shadows" and as The Warden in "Annie's Desert Adventure" (both 1954)
Glenn Strange, as Idaho in "Outlaw Brand" and as Ernie Barker in "Treasure Map" (both 1956)
Lyle Talbot, as Pa Wiggins in "Flint and Steel" and as Colonel Dawson in "Tagg Oakley, Sheriff" (both 1956)
Lee Van Cleef appeared as Tim Brennan in "Annie Breaks an Alibi" and as Amos Belcher in "Annie and the Higher Court" (both 1955).
Pierre Watkin, as Henry Lormier in "Dean Man's Bluff" (1955) and as the Reverend Mills in "The Reckless Press" (1956)
Carleton G. Young, as the Sheriff in "Sundown Stage" (1956) and as Colonel Jackson in "The Dutch Gunmaker" (1957)
Approximately two dozen episodes are currently available on DVD in North America (including several releases issued through Davis's estate) and elsewhere, although a full-series release has yet to happen.
On March 31, 2009, Mill Creek Entertainment released Gun Justice Featuring The Lone Ranger, with other television Westerns such as Annie Oakley.
A number of American comics were based on the TV series: