Nucleobase

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Base pairing in RNA. Nucleobases (C paired with G, A paired with U) in blue. Hydrogen bonds in red.
Chemical structure of DNA, showing the nitrogenous bases.

Nucleobases are nitrogen-containing biological compounds (nitrogenous bases) found within nucleosides—the basic building blocks of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Nucleobases are also found in nucleosides (a nucleobase linked to a sugar). Often simply called bases in genetics, their ability to form base-pairs and to stack upon one another lead directly to the helical structure of DNA and RNA.

Use of the word base is historical, in reference to the chemical properties of nucleobases in acid-base reactions within the test tube, and is not especially relevant or important for understanding most of their biological functions.

The primary nucleobases are cytosine (DNA and RNA), guanine (DNA and RNA), adenine (DNA and RNA), thymine (DNA) and uracil (RNA), abbreviated as C, G, A, T, and U, respectively. Because A, G, C, and T appear in the DNA, these molecules are called DNA-bases; A, G, C, and U are called RNA-bases. Uracil and thymine are identical except that uracil lacks the 5' methyl group. Adenine and guanine belong to the double-ringed class of molecules called purines (abbreviated as R). Cytosine, thymine, and uracil are all pyrimidines (abbreviated as Y).

In normal spiral DNA the bases form pairs between the two strands: A with T and C with G. Purines pair with pyrimidines mainly for dimensional reasons - only this combination fits the constant width geometry of the DNA spiral. The A-T and C-G pairings are required to match the hydrogen bonds between the amine and carbonyl groups on the complementary bases.

The compound formed when a nucleobase forms a glycosidic bond with the 1' anomeric carbon of a ribose or deoxyribose is called a nucleoside, and a nucleoside with one or more phosphate groups attached at the 5' carbon is called a nucleotide.

Apart from adenine (A), cytosine (C), guanine (G), thymine (T) and uracil (U), DNA and RNA also contain bases that have been modified after the nucleic acid chain has been formed. In DNA, the most common modified base is 5-methylcytosine (m5C). In RNA, there are many modified bases, including those contained in the nucleosides pseudouridine (Ψ), dihydrouridine (D), inosine (I), and 7-methylguanosine (m7G).[1][2]

Hypoxanthine and xanthine are two of the many bases created through mutagen presence, both of them through deamination (replacement of the amine-group with a carbonyl-group). Hypoxanthine is produced from adenine, xanthine from guanine.[3] In similar manner, deamination of cytosine results in uracil.

In August 2011, a report, based on NASA studies with meteorites found on Earth, was published suggesting nucleobases (such as adenine, guanine, xanthine, hypoxanthine, purine, 2,6-diaminopurine, and 6,8-diaminopurine) may have been formed in outer space.[4][5][6]

Structure[edit]

Primary bases[edit]

The following are nucleobases and ribonucleoside, a type of nucleoside including ribose as a component.

NucleobaseChemical structure of adenine
Adenine
Chemical structure of guanine
Guanine
Chemical structure of thymine
Thymine
Chemical structure of cytosine
Cytosine
Chemical structure of uracil
Uracil
RibonucleosideChemical structure of adenosine
Adenosine
A
Chemical structure of guanosine
Guanosine
G
Chemical structure of 5-methyluridine
5-Methyluridine
T
Chemical structure of cytidine
Cytidine
C
Chemical structure of uridine
Uridine
U

Modified purine bases[edit]

These are examples of modified adenosine or guanosine.

NucleobaseChemical structure of hypoxanthine
Hypoxanthine
Chemical structure of xanthine
Xanthine
Chemical structure of 7-methylguanine
7-Methylguanine
NucleosideChemical structure of inosine
Inosine
I
Chemical structure of xanthosine
Xanthosine
X
Chemical structure of 7-methylguanosine
7-Methylguanosine
m7G

Modified pyrimidine bases[edit]

These are examples of modified cytidine, thymidine or uridine.

NucleobaseChemical structure of dihydrouracil
5,6-Dihydrouracil
Chemical structure of 5-methylcytosine
5-Methylcytosine
Chemical structure of 5-hydroxymethylcytosine
5-Hydroxymethylcytosine
NucleosideChemical structure of dihydrouridine
Dihydrouridine
D
Chemical structure of 5-methylcytidine
5-Methylcytidine
m5C

Novel bases[edit]

A vast number of nucleobase analogues exist. The most common applications are used as fluorescent probes, either directly or indirectly, such as aminoallyl nucleotide, which are used to label cRNA or cDNA in microarrays. Several groups are working on alternative "extra" base pairs to extend the genetic code, such as isoguanine and isocytosine or the fluorescent 2-amino-6-(2-thienyl)purine and pyrrole-2-carbaldehyde.[citation needed]

In medicine, several nucleoside analogues are used as anticancer and antiviral agents. The viral polymerase incorporates these compounds with non-canon bases. These compounds are activated in the cells by being converted into nucleotides; they are administered as nucleosides as charged nucleotides cannot easily cross cell membranes.[citation needed] At least one set of new base pairs has been announced as of May 2014.[7]

See also[edit]

References[edit]

  1. ^ BIOL2060: Translation
  2. ^ "Role of 5' mRNA and 5' U snRNA cap structures in regulation of gene expression" - Research - Retrieved 13 December 2010.
  3. ^ T Nguyen, D Brunson, C L Crespi, B W Penman, J S Wishnok, and S R Tannenbaum, DNA damage and mutation in human cells exposed to nitric oxide in vitro, Proc Natl Acad Sci U S A. 1992 April 1; 89(7): 3030–3034
  4. ^ Callahan; Smith, K.E.; Cleaves, H.J.; Ruzica, J.; Stern, J.C.; Glavin, D.P.; House, C.H.; Dworkin, J.P. (11 August 2011). "Carbonaceous meteorites contain a wide range of extraterrestrial nucleobases". PNAS. doi:10.1073/pnas.1106493108. Retrieved 2011-08-15. 
  5. ^ Steigerwald, John (8 August 2011). "NASA Researchers: DNA Building Blocks Can Be Made in Space". NASA. Retrieved 2011-08-10. 
  6. ^ ScienceDaily Staff (9 August 2011). "DNA Building Blocks Can Be Made in Space, NASA Evidence Suggests". ScienceDaily. Retrieved 2011-08-09. 
  7. ^ D.A. Malyshev et al., “A semi-synthetic organism with an expanded genetic alphabet,” Nature, doi:10.1038/nature13314, 2014

External links[edit]